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Gene Expression Data
Assay Details
Assay
Reference: J:134028 Levesque BM, et al., NPAS1 regulates branching morphogenesis in embryonic lung. Am J Respir Cell Mol Biol. 2007 Apr;36(4):427-34
Assay type: RT-PCR
MGI Accession ID: MGI:7514385
Gene symbol: Sim2
Gene name: single-minded family bHLH transcription factor 2
Probe: Sim2 pF, Sim2 pR
Probe preparation: labelled with FAM
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Actb, and compared with levels at E11.5, which was defined as a unit level of one.
Results Image: 1a SIM2
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
E11.5 E11.5 TS19: lung Strong 0.2 µg; total RNA Swiss Webster Not Specified
E12.5 E12.5 TS20: lung Strong 0.2 µg; total RNA Swiss Webster Not Specified
E13.5 E13.5 TS21: lung Strong 0.2 µg; total RNA Swiss Webster Not Specified
E14.5 E14.5 TS22: lung Strong 0.2 µg; total RNA Swiss Webster Not Specified
E15.5 E15.5 TS23: lung Present 0.2 µg; total RNA Swiss Webster Not Specified
E16.5 E16.5 TS24: lung Present (a) 0.2 µg; total RNA Swiss Webster Not Specified
E17.5 E17.5 TS25: lung Present 0.2 µg; total RNA Swiss Webster Not Specified
E18.5 E18.5 TS26: lung Present (b) 0.2 µg; total RNA Swiss Webster Not Specified
P1 P1 TS27: lung Present (b) 0.2 µg; total RNA Swiss Webster Not Specified
not shown E10.5 TS17: lung Strong 0.2 µg; total RNA Swiss Webster Not Specified
Notes:
(a) Markedly declined at E16.5, and stayed down after.
(b) Lowest expression.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
01/06/2026
MGI 6.24
The Jackson Laboratory