Gpx3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:186220 Tondreau MY, et al., Sex-specific perinatal expression of glutathione peroxidases during mouse lung development. Mol Cell Endocrinol. 2012 May 15;355(1):87-95
Assay type:	RT-PCR
MGI Accession ID:	MGI:7511407
Gene symbol:	Gpx3
Gene name:	glutathione peroxidase 3

Probe:	Gpx3-pF, Gpx3-pR
Probe preparation:	labelled with SYBR green
Assay notes:	There were no significant differences in expression between males and females. Quantitative RT-PCR. Relative expression was normalized to Gapdh, Hprt1, and Sdha.

Results

Image: 2B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
GD15.5 male	E15.5	TS23: lung	Present	4 µg; total RNA	BALB/c		Male
GD15.5 female	E15.5	TS23: lung	Present	4 µg; total RNA	BALB/c		Female
GD17.5 male	E17.5	TS25: lung	Present	4 µg; total RNA	BALB/c		Male
GD17.5 female	E17.5	TS25: lung	Present	4 µg; total RNA	BALB/c		Female
GD19.5 male	E19.5	TS26: lung	Present	4 µg; total RNA	BALB/c		Male
GD19.5 female	E19.5	TS26: lung	Present	4 µg; total RNA	BALB/c		Female
PN0 male	P0	TS27: lung	Present	4 µg; total RNA	BALB/c		Male
PN0 female	P0	TS27: lung	Present	4 µg; total RNA	BALB/c		Female
PN1 male	P1	TS27: lung	Present	4 µg; total RNA	BALB/c		Male
PN1 female	P1	TS27: lung	Present	4 µg; total RNA	BALB/c		Female
PN2 male	P2	TS27: lung	Present	4 µg; total RNA	BALB/c		Male
PN2 female	P2	TS27: lung	Present	4 µg; total RNA	BALB/c		Female
PN3 male	P3	TS27: lung	Present	4 µg; total RNA	BALB/c		Male
PN3 female	P3	TS27: lung	Present	4 µg; total RNA	BALB/c		Female
PN5 male	P5	TS28: lung	Present	4 µg; total RNA	BALB/c		Male
PN5 female	P5	TS28: lung	Present	4 µg; total RNA	BALB/c		Female
PN7 male	P7	TS28: lung	Present	4 µg; total RNA	BALB/c		Male
PN7 female	P7	TS28: lung	Present	4 µg; total RNA	BALB/c		Female
PN15 male	P15	TS28: lung	Present (a)	4 µg; total RNA	BALB/c		Male
PN15 female	P15	TS28: lung	Present (a)	4 µg; total RNA	BALB/c		Female
PN20 male	P20	TS28: lung	Present	4 µg; total RNA	BALB/c		Male
PN20 female	P20	TS28: lung	Present	4 µg; total RNA	BALB/c		Female
PN30 male	P30	TS28: lung	Present (b)	4 µg; total RNA	BALB/c		Male
PN30 female	P30	TS28: lung	Present (b)	4 µg; total RNA	BALB/c		Female

Notes:
(a) Expression levels were increased by 4.2-fold from PN7 to PN15.
(b) The highest expression levels at PN30 are 58 times the levels at GD15.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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