Gene Expression Data
Assay Details
Assay
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Reference:
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J:198394
Ejarque M, et al., Neurogenin3 cooperates with Foxa2 to autoactivate its own expression. J Biol Chem. 2013 Apr 26;288(17):11705-17
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7510116
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Gene symbol:
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Foxa2
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Gene name:
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forkhead box A2
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Results
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Specimen
5A/B/C:
embryonic day 15.5
(more )
Note: Triple labeled: green - Foxa2; red - Neurog3 (assay MGI:7510117); blue - Sox9 (assay MGI:7510118).
Specimen
5A/B/C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Foxa2; red - Neurog3 (assay MGI:7510117); blue - Sox9 (assay MGI:7510118).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: pancreas epithelium |
Present |
Widespread |
5A
,
5B
,
5C
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Expressed at lower levels in the cells lining the lumen of the epithelial chords, while high level cells were detected within or adjacent to the epithelial chords. There was a statistically significant correlation between Neurog3 and Foxa2 expression levels (see Fig 5D and 5E).
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Specimen
S3A:
embryonic day 13.5
(more )
Specimen
S3A:
(close )
Genetic Background:
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CD-1
|
Age:
|
embryonic day 13.5
|
Sex:
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Not Specified
|
Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: pancreas epithelium |
Present |
Widespread |
S3A
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Homogeneously expressed throughout the branching pancreatic epithelium, with the exception of a few cells displaying higher levels.
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Specimen
S3B:
embryonic day 14.5
(more )
Specimen
S3B:
(close )
Genetic Background:
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CD-1
|
Age:
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embryonic day 14.5
|
Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pancreas epithelium |
Present |
Widespread |
S3B
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Expressed at lower levels in the cells lining the lumen of the epithelial chords, while high level cells were detected within or adjacent to the epithelial chords.
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Specimen
S3C/E:
embryonic day 13.5
(more )
Note: Double labeled: green - Foxa2; red - Neurog3 (assay MGI:7510117).
Specimen
S3C/E:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Foxa2; red - Neurog3 (assay MGI:7510117).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: pancreas epithelium |
Present |
|
S3E
,
S3C
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Cells with higher expression appeared both grouped in clusters or as single cells within or in close proximity to the pancreatic epithelial chords.
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Specimen
S3D/F:
embryonic day 14.5
(more )
Note: Double labeled: green - Foxa2; red - Neurog3 (assay MGI:7510117).
Specimen
S3D/F:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
Double labeled: green - Foxa2; red - Neurog3 (assay MGI:7510117).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pancreas epithelium |
Present |
|
S3F
,
S3D
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Expressed at lower levels in the cells lining the lumen of the epithelial chords, while high level cells were detected within or adjacent to the epithelial chords.
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Specimen
S4A:
embryonic day 14.5
(more )
Note: Double labeled: green - Foxa2; red - Sox9 (assay MGI:7510119).
Specimen
S4A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Foxa2; red - Sox9 (assay MGI:7510119).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pancreas epithelium |
Present |
|
S4A
|
High-level Foxa2 expression cells did not express Sox9. Coexpressed with Sox9 in low-level Foxa2 expression cells.
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Specimen
S4B:
embryonic day 14.5
(more )
Note: Double labeled: green - Foxa2; red - Muc1 (assay MGI:7510120).
Specimen
S4B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Foxa2; red - Muc1 (assay MGI:7510120).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pancreas epithelium |
Present |
|
S4B
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Low-level Foxa2 expression cells, lining the epithelial chords, coexpressed Muc1.
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Specimen
S4C:
embryonic day 14.5
(more )
Note: Double labeled: green - Foxa2; red - Nkx6-1 (assay MGI:7510121).
Specimen
S4C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
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Double labeled: green - Foxa2; red - Nkx6-1 (assay MGI:7510121).
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Specimen
S4D:
embryonic day 14.5
(more )
Note: Double labeled: green - Foxa2; red - Pdx1 (assay MGI:7510122).
Specimen
S4D:
(close )
Genetic Background:
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CD-1
|
Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
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Double labeled: green - Foxa2; red - Pdx1 (assay MGI:7510122).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pancreas epithelium |
Present |
|
S4D
|
Almost complete overlap between Foxa2 and Pdx1 expression. Most of the Pdx1 highly expressed cells did not display the highest levels of Foxa2.
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Specimen
S4E:
embryonic day 14.5
(more )
Note: Double labeled: green - Foxa2; red - Ins1 (assay MGI:7510123) and Ins2 (assay MGI:7511621).
Specimen
S4E:
(close )
Genetic Background:
|
CD-1
|
Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Foxa2; red - Ins1 (assay MGI:7510123) and Ins2 (assay MGI:7511621).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pancreas epithelium |
Present |
|
S4E
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Most of the Foxa2 highly expressed cells were Ins1/2 negative (not beta-cells).
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Specimen
S4F:
embryonic day 14.5
(more )
Note: Double labeled: green - Foxa2; red - Chga (assay MGI:7510124).
Specimen
S4F:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
Double labeled: green - Foxa2; red - Chga (assay MGI:7510124).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pancreas epithelium |
Present |
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S4F
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Almost complete overlap between Foxa2 highly expressed cells and Chga expression, showing their likely specification toward an endocrine fate.
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