Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:321845
Bernhardt N, et al., Hop Mice Display Synchronous Hindlimb Locomotion and a Ventrally Fused Lumbar Spinal Cord Caused by a Point Mutation in Ttc26. eNeuro. 2022 Mar-Apr;9(2):ENEURO.0518-21.2022
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:7509794
|
|
Gene symbol:
|
Isl1
|
|
Gene name:
|
ISL1 transcription factor, LIM/homeodomain
|
|
Antibody:
|
anti-Isl1/2
|
|
Assay notes:
|
Two Rbfox3 antibodies were described, but the one used in this assay was not detailed. Anti-NeuN MGI:7509750 may have been used in this assay.
|
|
|
Results
|
Specimen
S4E/E':
embryonic day 12.5
(more  )
Specimen
S4E/E':
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Embedding:
|
Cryosection
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
spinal cord interneuron |
Present |
Regionally restricted |
S4/ED6-1E'
,
S4/ED6-1E
|
Expression in dl3 interneurons and in motor neurons on and close to ventral midline not seen in controls
|
| TS20: spinal cord |
spinal cord motor neuron |
Present |
Regionally restricted |
S4/ED6-1E'
,
S4/ED6-1E
|
Expression in dl3 interneurons and in motor neurons on and close to ventral midline not seen in controls
|
Specimen
S4J/J':
(close )
|
Genetic Background:
|
involves: BALB/cByJ * C57BL/6J
|
|
Mutant Allele(s):
|
Ift56hop/Ift56hop
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Embedding:
|
Cryosection
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
spinal cord interneuron |
Present |
Regionally restricted |
S4/ED6-1J'
,
S4/ED6-1J
|
Expression in dl3 interneurons and in motor neurons on and close to ventral midline.
|
| TS20: spinal cord |
spinal cord motor neuron |
Present |
Regionally restricted |
S4/ED6-1J'
,
S4/ED6-1J
|
Expression in dl3 interneurons and in motor neurons on and close to ventral midline.
|
|
Analysis Tools
