Gene Expression Data
Assay Details
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Assay
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Reference:
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J:321845
Bernhardt N, et al., Hop Mice Display Synchronous Hindlimb Locomotion and a Ventrally Fused Lumbar Spinal Cord Caused by a Point Mutation in Ttc26. eNeuro. 2022 Mar-Apr;9(2):ENEURO.0518-21.2022
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7509784
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Antibody:
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anti-Shh
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Assay notes:
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Two Rbfox3 antibodies were described, but the one used in this assay was not detailed. Anti-NeuN MGI:7509750 may have been used in this assay.
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Results
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Specimen
6A:
embryonic day 12.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Embedding:
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Cryosection
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Specimen
6A':
embryonic day 12.5
(more )
Specimen
6A':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Embedding:
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Cryosection
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Specimen
6A'':
embryonic day 12.5
(more )
Specimen
6A'':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Embedding:
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Cryosection
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Specimen
6B:
(close )
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Genetic Background:
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involves: BALB/cByJ * C57BL/6J
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Mutant Allele(s):
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Ift56hop/Ift56hop
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: notochord |
Trace |
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6B
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Distance between spinal cord and notochord decreased compared to control.
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| TS20: spinal cord |
Trace |
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6B
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Distance between spinal cord and notochord decreased compared to control.
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Specimen
6B':
(close )
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Genetic Background:
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involves: BALB/cByJ * C57BL/6J
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Mutant Allele(s):
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Ift56hop/Ift56hop
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: notochord |
Trace |
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6B'
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Expression in notochord reduced compared to control. Low levels of expression. Abnormal notochord morphology with disorganized cells.
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Specimen
6B'':
(close )
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Genetic Background:
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involves: BALB/cByJ * C57BL/6J
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Mutant Allele(s):
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Ift56hop/Ift56hop
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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optical section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord |
Trace |
Regionally restricted |
6B''
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Expression in spinal cord reduced compared to control. Expression almost absent in ventral spinal cord.
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Specimen
6C:
embryonic day 10.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Embedding:
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Cryosection
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Specimen
6D:
(close )
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Genetic Background:
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involves: BALB/cByJ * C57BL/6J
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Mutant Allele(s):
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Ift56hop/Ift56hop
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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optical section
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Embedding:
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Cryosection
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Analysis Tools