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Gene Expression Data
Assay Details
Assay
Reference: J:178471 Rumballe BA, et al., Nephron formation adopts a novel spatial topology at cessation of nephrogenesis. Dev Biol. 2011 Dec 1;360(1):110-22
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7509577
Gene symbol: Six2
Gene name: sine oculis-related homeobox 2
Antibody: anti-SIX2 (H00010736-M01), clone 3D7
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 3/S2 E15.5: embryonic day 15.5 (more )
Note: Double labeled: red - Six2; green - Collagen IV.
Structure Level Pattern Image Note
TS23: pretubular aggregate Weak S2_Six2 E15.5 , 3a
TS23: renal vesicle Weak S2_Six2 E15.5 , 3a
TS23: cap mesenchyme Strong 3a , S2_Six2 E15.5
Specimen 3/S2 P0: postnatal day 0 (more )
Note: Double labeled: red - Six2; green - Collagen IV.
Structure Level Pattern Image Note
TS27: pretubular aggregate Weak S2_Six2 P0 , 3b
TS27: renal vesicle Weak S2_Six2 P0 , 3b
TS27: cap mesenchyme Strong S2_Six2 P0 , 3b
Specimen 3/S2 P2: postnatal day 2 (more )
Note: Double labeled: red - Six2; green - Collagen IV.
Structure Level Pattern Image Note
TS27: renal vesicle Strong S2_Six2 P2 , 3c
Specimen 3 P4: postnatal day 4 (more )
Note: Double labeled: red - Six2; green - Collagen IV.
Structure Level Pattern Image Note
TS28: metanephros Absent 3d
Specimen not shown: embryonic day 15.5 (more )
Note: Summarized in Fig 8e. Triple labeled: red - Six2; green - Calb1 (assay 7509604); white - pHH3.
Structure Level Pattern Image Note
TS23: cap mesenchyme Present
Specimen not shown: postnatal day 0 (more )
Note: Summarized in Fig 8e. Triple labeled: red - Six2; green - Calb1 (assay 7509604); white - pHH3.
Structure Level Pattern Image Note
TS27: cap mesenchyme Present Reduced total positive cell numbers, percentage of the positive cells in mitosis and the number of positive cells per tip compared with E15.5.
Specimen not shown: postnatal day 2 (more )
Note: Summarized in Fig 8e. Triple labeled: red - Six2; green - Calb1 (assay 7509604); white - pHH3.
Structure Level Pattern Image Note
TS27: cap mesenchyme Present Reduced total positive cell numbers, percentage of the positive cells in mitosis and the number of positive cells per tip compared with E15.5. Reduced number of positive cells per tip compared with P0.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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