Lgr5 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:212415 Kinzel B, et al., Functional roles of Lgr4 and Lgr5 in embryonic gut, kidney and skin development in mice. Dev Biol. 2014 Jun 15;390(2):181-90
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7509432
Gene symbol:	Lgr5
Gene name:	leucine rich repeat containing G protein coupled receptor 5

Reporter:	GFP
Antibody:	anti-GFP (ab6673)
Detection system:	Secondary antibody

Results

2B bottom ©

2B top ©

Specimen 2B: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine	Present	Single cells	2B bottom , 2B top	Expression is in a few cells in the developing intestinal crypt base.

3A Lgr5^EGFP ©

Specimen 3A Lgr5^EGFP: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine	Present	Single cells	3A Lgr5^EGFP	Expression is in a few stem cells in the developing intestinal crypt base.

3A Lgr5KO ©

Specimen 3A Lgr5KO: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine	Present	Single cells	3A Lgr5KO	Expression is in a few stem cells in the developing intestinal crypt base.

3A Lgr4KO;Lgr5^EGFP ©

Specimen 3A Lgr4KO;Lgr5^EGFP: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine	Absent		3A Lgr4KO;Lgr5^EGFP

3A Lgr4/5dKO ©

Specimen 3A Lgr4/5dKO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: intestine	Absent		3A Lgr4/5dKO

4B ©

Specimen 4B: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: hair follicle		Present		4B
TS24: dermis	fibroblast of dermis	Present		4B	Expression is in dermal fibroblasts.

4J Lgr5^EGFP ©

Specimen 4J Lgr5^EGFP: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Present		4J Lgr5^EGFP

4J Lgr4KO;Lgr5^EGFP ©

Specimen 4J Lgr4KO;Lgr5^EGFP: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Present		4J Lgr4KO;Lgr5^EGFP

4J Lgr5KO ©

Specimen 4J Lgr5KO: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Present		4J Lgr5KO

4J Lgr4/5dKO ©

Specimen 4J Lgr4/5dKO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Absent		4J Lgr4/5dKO

5B left ©

5B right ©

Specimen 5B: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Present	Regionally restricted	5B right , 5B left	Expression is in the tubular epithelium.
TS24: Bowman's capsule of maturing renal corpuscle	Absent		5B right , 5B left

5G Lgr5^EGFP ©

Specimen 5G Lgr5^EGFP: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Present	Regionally restricted	5G Lgr5^EGFP	Expression is in the tubular epithelium.

Specimen 5G Lgr4KO;Lgr5^EGFP: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Present	Regionally restricted	5G Lgr4KO;Lgr5^EGFP	Expression is in the tubular epithelium.

5G Lgr5KO ©

Specimen 5G Lgr5KO: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Present	Regionally restricted	5G Lgr5KO	Expression is in the tubular epithelium.

5G Lgr4/5dKO ©

Specimen 5G Lgr4/5dKO: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5^{tm1.1(cre/ERT2)Npa} (more

)

Structure	Level	Pattern	Image	Note
TS24: renal tubule	Present	Regionally restricted	5G Lgr4/5dKO	Expression is in the tubular epithelium.

Specimen S6A Lgr5^EGFP: embryonic day 16.5; Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: dermis	fibroblast of dermis	Present	Regionally restricted	S6A Lgr5^EGFP	Expression is in dermal fibroblasts.

Specimen S6A Lgr4KO; Lgr5^EGFP: embryonic day 16.5; Lgr4^{tm1.1(cre/ERT2)Npa}/Lgr4^{tm1.1(cre/ERT2)Npa}, Lgr5^{tm1.1(cre/ERT2)Npa}/Lgr5⁺ (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS24: dermis	fibroblast of dermis	Present	Regionally restricted	S6A Lgr4KO;Lgr5^EGFP	Expression is in dermal fibroblasts.

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