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Gene Expression Data
Assay Details
Assay
Reference: J:165898 Suzuki-Inoue K, et al., Essential in vivo roles of the C-type lectin receptor CLEC-2: embryonic/neonatal lethality of CLEC-2-deficient mice by blood/lymphatic misconnections and impaired thrombus formation of CLEC-2-deficient platelets. J Biol Chem. 2010 Aug 6;285(32):24494-507
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7495701
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2B +/+: embryonic day 17.5 (more )
Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:7495702); blue - Lyve1 (assay MGI:7495700).
Structure Cell Type Level Pattern Image Note
TS25: artery endothelium endothelial cell of artery Present 2B +/+ PECAM-1 , 2B +/+ Merge
TS25: vein endothelium vein endothelial cell Present 2B +/+ PECAM-1 , 2B +/+ Merge
TS25: lymphatic vessel endothelium endothelial cell of lymphatic vessel Present 2B +/+ Merge , 2B +/+ PECAM-1 There were no blood cells in the lymphatic vessels.
Specimen 2B -/-: embryonic day 17.5; Clec1btm1.1Ksi/Clec1btm1.1Ksi (more )
Note: Triple labeled: green - Pecam1; red - Acta2 (assay MGI:7495702); blue - Lyve1 (assay MGI:7495700).
Structure Cell Type Level Pattern Image Note
TS25: vein endothelium vein endothelial cell Present 2B -/- Merge , 2B -/- PECAM-1
TS25: artery endothelium endothelial cell of artery Present 2B -/- Merge , 2B -/- PECAM-1
TS25: lymphatic vessel endothelium endothelial cell of lymphatic vessel Present 2B -/- PECAM-1 , 2B -/- Merge Peripheral blood cells were present within the lymphatic vessels.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory