Gene Expression Data
Assay Details
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Assay
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Reference:
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J:330741
Orchard P, et al., Genome-wide chromatin accessibility and transcriptome profiling show minimal epigenome changes and coordinated transcriptional dysregulation of hedgehog signaling in Danforth's short tail mice. Hum Mol Genet. 2019 Mar 1;28(5):736-750
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7491736
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Antibody:
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5E1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
5A:
embryonic day 10.5
(more  )
Note: Level of the forelimb.
Specimen
5A:
(close  )
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Genetic Background:
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involves: CD-1 * Danforth's posterior duplication stock
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Level of the forelimb.
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Specimen
5B:
embryonic day 10.5; Etn2Sd/Etn2 +
(more )
Note: Level of the forelimb.
Specimen
5B:
(close )
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Genetic Background:
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involves: CD-1 * Danforth's posterior duplication stock * SD
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Mutant Allele(s):
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Etn2Sd/Etn2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Level of the forelimb.
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Specimen
5C:
embryonic day 10.5; Etn2Sd/ Etn2Sd
(more )
Note: Level of the forelimb.
Specimen
5C:
(close )
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Genetic Background:
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involves: CD-1 * Danforth's posterior duplication stock * SD
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Level of the forelimb.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube floor plate |
Present |
Regionally restricted |
5C
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In this mutant the notochord is absent but floor plate expression remained at the level of the forelimb (shown) and is absent at the level of the hindlimb.
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Specimen
5D:
embryonic day 10.5
(more )
Note: Level of the hindlimb.
Specimen
5D:
(close )
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Genetic Background:
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involves: CD-1 * Danforth's posterior duplication stock
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Level of the hindlimb.
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Specimen
5E:
embryonic day 10.5; Etn2Sd/Etn2 +
(more )
Note: Level of the hindlimb.
Specimen
5E:
(close )
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Genetic Background:
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involves: CD-1 * Danforth's posterior duplication stock * SD
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Mutant Allele(s):
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Etn2Sd/Etn2+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Level of the hindlimb.
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Specimen
5F:
embryonic day 10.5; Etn2Sd/ Etn2Sd
(more )
Note: Level of the hindlimb.
Specimen
5F:
(close )
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Genetic Background:
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involves: CD-1 * Danforth's posterior duplication stock * SD
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Mutant Allele(s):
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Etn2Sd/Etn2Sd
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Level of the hindlimb.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube floor plate |
Present |
Regionally restricted |
5F
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In this mutant the notochord is absent but floor plate expression remained at the level of the forelimb and is absent at the level of the hindlimb (shown).
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Analysis Tools
