Probe:	Chd8 probe
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Widespread	1A	Expression in most embryonic tissues.
TS20: central nervous system	Present	Regionally restricted	1A	Expression throughout neuroepithelium of developing central nervous system.
TS20: cerebellum primordium	Present		1A
TS20: neocortex	Present		1A
TS20: hindbrain floor plate	Present		1A
TS20: lower rhombic lip	Present		1A
TS20: dorsal root ganglion	Present		1A
TS20: spinal cord	Present		1A
TS20: diencephalon	Strong		1A
TS20: hypothalamus	Strong		1A
TS20: pituitary gland	Strong		1A
TS20: olfactory epithelium	Present		1A
TS20: tongue	Present		1A
TS20: paraxial mesenchyme	Present	Regionally restricted	1A	Expression in intersomitic mesoderm
TS20: genital tubercle	Present		1A
TS20: gut	Present		1A
TS20: lung	Present		1A
TS20: tail	Present		1A

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present	Regionally restricted	1Aa	Expression in ventricular zone.
TS20: rhombic lip	Present		1Aa
TS20: midbrain-hindbrain junction	Present		1Aa	Expression in isthmus.

Structure	Level	Pattern	Image	Note
TS20: neocortex	Present	Widespread	1Ab
TS20: cerebral cortex subventricular zone	Present	Widespread	1Ab	Expression throughout ventricular and subventricular regions of neocortex.
TS20: cerebral cortex ventricular layer	Present	Widespread	1Ab	Expression throughout ventricular and subventricular regions of neocortex.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Widespread	S1A	Expression throughout length of neural tube.
TS20: dorsal root ganglion	Present		S1A

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Widespread	S1B	Expression throughout length of neural tube.
TS20: paraxial mesenchyme	Present	Regionally restricted	S1B	Expression in intersomitic regions.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		S1C	No mediolateral or dorsoventral gradient.
TS20: dorsal root ganglion	Present		S1C
TS20: eye	Present		S1C

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		S1D	No mediolateral or dorsoventral gradient.
TS20: dorsal root ganglion	Present		S1D

Structure	Level	Pattern	Image	Note
TS20: cochlear duct epithelium	Present		2A
TS20: vestibulo-cochlear ganglion	Present		2A

Structure	Level	Pattern	Image	Note
TS20: metanephros	Strong	Regionally restricted	2B	High expression in mesenchyme and metanephric tubule epithelium.
TS20: cap mesenchyme	Present		2B

Structure	Level	Pattern	Image	Note
TS20: lung epithelium	Present		2C

Structure	Level	Pattern	Image	Note
TS20: neural retina	Present		2D
TS20: pigmented retinal epithelium	Present		2D
TS20: head mesenchyme	Present	Widespread	2D	Expression present widely throughout surrounding mesenchyme and craniofacial tissues.

Structure	Level	Pattern	Image	Note
TS20: tooth bud	Strong	Regionally restricted	2E	High expression in incisor primordium.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Regionally restricted	3A	Distinct expression patterns throughout embryo.
TS22: nervous system	Strong	Regionally restricted	3A	Notably higher levels in developing nervous system.
TS22: forebrain	Strong	Regionally restricted	3A	Prominent expression in basal forebrain.
TS22: cerebellum	Present		3A
TS22: neocortex	Present		3A
TS22: diencephalon	Weak	Diffuse	3A
TS22: midbrain	Weak	Diffuse	3A
TS22: spinal cord	Weak		3A
TS22: incisor	Present		3A
TS22: olfactory epithelium	Present		3A
TS22: tongue	Present		3A
TS22: gut	Present		3A
TS22: lung	Present		3A
TS22: metanephros	Present		3A
TS22: heart	Present		3A
TS22: hindlimb digit	Present		3A
TS22: liver	Present		3A
TS22: stomach epithelium	Present		3A
TS22: thymus primordium	Present		3A
TS22: thyroid gland	Present		3A
TS22: trigeminal ganglion	Present		3A

Structure	Level	Pattern	Image	Note
TS22: cerebellum ventricular layer	Strong		3Aa
TS22: rhombic lip	Strong		3Aa

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex mantle layer	Present	Regionally restricted	3Ab
TS22: cerebral cortex subventricular zone	Present	Regionally restricted	3Ab
TS22: cerebral cortex ventricular layer	Present	Regionally restricted	3Ab
TS22: neocortex	Present	Regionally restricted	3Ab

Structure	Level	Pattern	Image	Note
TS22: lens	Present		3Ac
TS22: neural retina	Present		3Ac

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present		3Ad

Structure	Level	Pattern	Image	Note
TS22: cap mesenchyme	Present		3Ae	Expression in condensing mesenchyme of kidney.

Structure	Level	Pattern	Image	Note
TS27: brain	Present	Widespread	4A
TS27: hippocampus	Present		4A
TS27: hypothalamus	Present		4A
TS27: main olfactory bulb	Present		4A

Structure	Level	Pattern	Image	Note
TS27: brain	Present	Widespread	4A'
TS27: hippocampus	Present		4A'
TS27: hypothalamus	Present		4A'
TS27: main olfactory bulb	Present		4A'

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Moderate	Diffuse	4Aa

Structure	Level	Pattern	Image	Note
TS27: neocortex	Present	Regionally restricted	4Ab	Slight enrichment of expression towards outer neocortex.

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Widespread	5A
TS28: cerebellum	Present		5A
TS28: hippocampus	Present		5A
TS28: neocortex	Present		5A

Structure	Level	Pattern	Image	Note
TS28: brain	Present	Widespread	5A'
TS28: cerebellum	Present		5A'
TS28: hippocampus	Present		5A'
TS28: main olfactory bulb	Present		5A'
TS28: neocortex	Present		5A'
TS28: rostral migratory stream	Present		5A'

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Strong		5Aa

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		5Ab
TS28: hippocampus CA1	Present		5Ab
TS28: hippocampus CA2	Present		5Ab
TS28: hippocampus CA3	Present		5Ab

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		5Ac
TS28: cortical layer III	Present		5Ac

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		6A
TS28: hippocampus	Present		6A
TS28: neocortex	Present		6A
TS28: rostral migratory stream	Present		6A

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present		6A'
TS28: main olfactory bulb	Present		6A'
TS28: neocortex	Present		6A'

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present		6Aa

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		6Ab
TS28: hippocampus CA1	Present		6Ab
TS28: hippocampus CA2	Present		6Ab
TS28: hippocampus CA3	Present		6Ab

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present		6Ac