Antibody:	anti-Calb1 (clone CB-955) (C9848; AM08219SU-N)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum vermis	Purkinje cell	Present		2A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum vermis	Purkinje cell	Present		2B	No signficant difference in number of Purkinje cells compared to wild type.

Structure	Level	Pattern	Image	Note
TS28: cerebellum vermis	Present		2C
TS28: deep cerebellar nucleus	Present		2C

Structure	Cell Type	Level	Pattern	Image	Note
TS28: cerebellum anterior vermis	Purkinje cell	Weak	Regionally restricted	2D	Progressive loss of Purkinje cells particularly in anterior and posterior lobes of vermis.
TS28: cerebellum posterior vermis	Purkinje cell	Weak	Regionally restricted	2D	Progressive loss of Purkinje cells particularly in anterior and posterior lobes of vermis.
TS28: deep cerebellar nucleus		Present		2D	Authors report progressive neurodegeneration in mutant.