Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:297659
Ge Y, et al., Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins. Dev Dyn. 2020 Aug 2;
|
Assay type:
|
In situ reporter (transgenic)
|
MGI Accession ID:
|
MGI:7486122
|
Gene symbol:
|
Atoh7
|
Gene name:
|
atonal bHLH transcription factor 7
|
Reporter:
|
Cre
|
Antibody:
|
anti-cre
|
Assay notes:
|
The pregnant mice were intraperitoneally injected of tamoxifen at E14.5 and assayed at indicated time after the administration.
|
|
Results
|
Specimen
6B:
embryonic day 14.5; Atoh7tm3(cre/ERT2)Xmu/Atoh7 +
(more )
Note: at 1 hour after tamoxifen treatment
Specimen
6B:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6NCrl
|
Mutant Allele(s):
|
Atoh7tm3(cre/ERT2)Xmu/Atoh7+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
at 1 hour after tamoxifen treatment
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: neural retina |
Present |
Regionally restricted |
6B
|
Expression is seen in cells in the neuroblast layer; due to exposure to tamoxifen, partial nuclear location of the Cre protein is seen.
|
Specimen
6C:
embryonic day 14.5; Atoh7tm3(cre/ERT2)Xmu/Atoh7 +
(more )
Note: at 2 hours afters tamoxifen treatment
Specimen
6C:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * C57BL/6NCrl
|
Mutant Allele(s):
|
Atoh7tm3(cre/ERT2)Xmu/Atoh7+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
at 2 hours afters tamoxifen treatment
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: neural retina |
Present |
|
6C
|
Expression is seen in cells in the neuroblast layer; due to exposure to tamoxifen, mainly nuclear location of the Cre protein is seen.
|
|