Gene Expression Data
Assay Details
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Assay
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Reference:
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J:135242
Kala K, et al., Analysis of the midbrain-hindbrain boundary cell fate using a boundary cell-specific Cre-mouse strain. Genesis. 2008 Jan;46(1):29-36
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7485828
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Gene symbol:
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Cdkn1a
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Gene name:
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cyclin dependent kinase inhibitor 1A
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Antibody:
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anti-p21
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3b:
embryonic day 11.5
(more  )
Note: Coronal section.
Specimen
3b:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Regionally restricted |
3b
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Expression is seen in the most posterior midbrain and the most anterior rhombomere 1 (r1) of hindbrain.
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| TS19: midbrain |
Present |
Regionally restricted |
3b
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Expression is seen in the most posterior midbrain and the most anterior rhombomere 1 (r1) of hindbrain.
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Specimen
3d:
embryonic day 11.5; Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor +, Tg(Hoxb7-cre)1Jpa/0
(more )
Note: Conditional mutant. Coronal section. Double labeled: green - Cdkn1a; red - Cre reporter.
Specimen
3d:
(close )
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Genetic Background:
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involves: 129S4/SvJaeSor * C57BL/6
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Mutant Allele(s):
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Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Tg(Hoxb7-cre)1Jpa/0
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant. Coronal section. Double labeled: green - Cdkn1a; red - Cre reporter.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Regionally restricted |
3d
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Expression is seen in the most posterior midbrain and the most anterior rhombomere 1 (r1) of hindbrain.
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| TS19: midbrain |
Present |
Regionally restricted |
3d
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Expression is seen in the most posterior midbrain and the most anterior rhombomere 1 (r1) of hindbrain.
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Specimen
3f/g:
embryonic day 11.5
(more )
Note: Coronal section. Double labeled: green - Cdkn1a; red - BrdU.
Specimen
3f/g:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Double labeled: green - Cdkn1a; red - BrdU.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Single cells |
3f
,
3g
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Expression is seen in the most posterior midbrain and the most anterior rhombomere 1 (r1) of hindbrain. These cells are not actively progressing in the cell cycle.
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| TS19: midbrain |
Present |
Single cells |
3f
,
3g
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Expression is seen in the most posterior midbrain and the most anterior rhombomere 1 (r1) of hindbrain. These cells are not actively progressing in the cell cycle.
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Specimen
not shown:
embryonic day 10.5
(more )
Note: Also labeled for BrdU.
Specimen
not shown:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Also labeled for BrdU.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Single cells |
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Expression is seen in the most posterior midbrain and the most anterior rhombomere 1 (r1) of hindbrain. These cells are not actively progressing in the cell cycle.
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| TS17: rhombomere 1 |
Present |
Single cells |
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Expression is seen in the most posterior midbrain and the most anterior rhombomere 1 (r1) of hindbrain. These cells are not actively progressing in the cell cycle.
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Specimen
3i:
embryonic day 11.5
(more )
Note: Coronal section. Double labeled: green - Cdkn1a; red - Mki67 (assay MGI:7485886).
Specimen
3i:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Double labeled: green - Cdkn1a; red - Mki67 (assay MGI:7485886).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Single cells |
3i
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Expression is seen in the most posterior midbrain and the most anterior rhombomere 1 (r1) of hindbrain. These cells are not actively progressing in the cell cycle.
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| TS19: midbrain |
Present |
Single cells |
3i
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Expression is seen in the most posterior midbrain and the most anterior rhombomere 1 (r1) of hindbrain. These cells are not actively progressing in the cell cycle.
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Specimen
3m:
embryonic day 11.5
(more )
Note: Coronal section. Double labeled: green - Cdkn1a; red - Mki67 (assay MGI:7485886).
Specimen
3m:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Double labeled: green - Cdkn1a; red - Mki67 (assay MGI:7485886).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Single cells |
3m
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Expression is seen in the most posterior midbrain and the most anterior rhombomere 1 (r1) of hindbrain. These cells are not actively progressing in the cell cycle.
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| TS19: midbrain |
Present |
Single cells |
3m
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Expression is seen in the most posterior midbrain and the most anterior rhombomere 1 (r1) of hindbrain. These cells are not actively progressing in the cell cycle.
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Analysis Tools