Gene Expression Data
Assay Details
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Assay
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Reference:
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J:335331
Seppala M, et al., Gas1 Regulates Patterning of the Murine and Human Dentitions through Sonic Hedgehog. J Dent Res. 2022 Apr;101(4):473-482
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7481961
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Gene symbol:
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Spry2
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Gene name:
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sprouty RTK signaling antagonist 2
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Probe:
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Sprouty2 probe
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Probe preparation:
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labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3U:
embryonic day 13.5
(more  )
Specimen
3U:
(close  )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: upper jaw mesenchyme |
Present |
Regionally restricted |
3U
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Expression in vestigial tooth primordium (R2).
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Specimen
3V:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Gas1tm1Fan/Gas1tm1Fan
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: upper jaw mesenchyme |
Strong |
Regionally restricted |
3V
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Expression increased in R2 compared to wild type.
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Specimen
3Wi:
embryonic day 14.5
(more )
Specimen
3Wi:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3Wii:
embryonic day 14.5
(more )
Specimen
3Wii:
(close )
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Genetic Background:
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involves: 129 * C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: enamel knot |
Present |
Regionally restricted |
3Wii
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Expression in more lingual regions of tooth germ in association with 1st molar enamel knot.
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| TS22: upper jaw molar |
Present |
Regionally restricted |
3Wii
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Expression in more lingual regions of tooth germ in association with 1st molar enamel knot.
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Specimen
3X:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6
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Mutant Allele(s):
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Gas1tm1Fan/Gas1tm1Fan
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: upper jaw mesenchyme |
Strong |
Regionally restricted |
3X
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Expression increased in R2 compared to wild type.
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Analysis Tools