Trp53 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:207609 Hilliard SA, et al., Mdm2 is required for maintenance of the nephrogenic niche. Dev Biol. 2014 Mar 1;387(1):1-14
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7481337
Gene symbol:	Trp53
Gene name:	transformation related protein 53

Antibody:	anti-p53 (CM5, P53-PROTEIN-CM5)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

4A ©

Specimen 4A: embryonic day 13.5 (more

)
Note: Double labeled: green - Trp53; red - GFP (from Cre transgene)

Structure	Level	Pattern	Image	Note
TS21: metanephros	Ambiguous		4A

4B ©

Specimen 4B: embryonic day 13.5; Mdm2^tm1.2Mep/Mdm2^tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more

)
Note: Conditional mutant. Double labeled: green - Trp53; red - GFP (from Cre transgene)

Structure	Level	Pattern	Image	Note
TS21: cap mesenchyme	Present		4B	Increased expression specifically in the cap mesenchyme cells which stained positively for Six2 promoter driven GFP.

Specimen not shown: embryonic day 13.5; Mdm2^tm1.2Mep/Mdm2^tm1.2Mep, Tg(Six2-GFP/cre)1Amc/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS21: early nephron	Present			Increased expression in the cells of the nascent nephrons derived from the cap.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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