Antibody:	anti-Pold2 (PA5-55401)
Detection system:	Secondary antibody coupled to Alexa Fluor 546

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present		2c GV	Expression is concentrated in the nuclei of germinal vesicle stage oocytes.

Structure	Level	Pattern	Image	Note
TS28: secondary oocyte	Present		2c MII	Unlike at other early stages, in metaphase II oocytes expression is not concentrated in the nuclei.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		2c Zy	Expression is concentrated in the nuclei

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		2c 2C	Expression is concentrated in the nuclei

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present		2c 4C	Expression is concentrated in the nuclei

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		2c 8C	Expression is concentrated in the nuclei

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		2c Mo	Expression is concentrated in the nuclei

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present	Widespread	2c Bl	Expression is seen in all cells at the blastocyst stage, without obvious difference among distinct cell lineages.
TS4: trophectoderm	Present	Widespread	2c Bl	Expression is seen in all cells at the blastocyst stage, without obvious difference among distinct cell lineages.