Gli3 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:172775 Gildea DE, et al., The pleiotropic mouse phenotype extra-toes spotting is caused by translation initiation factor Eif3c mutations and is associated with disrupted sonic hedgehog signaling. FASEB J. 2011 May;25(5):1596-605
Assay type:	Western blot
MGI Accession ID:	MGI:7467467
Gene symbol:	Gli3
Gene name:	GLI-Kruppel family member GLI3

Antibody:	Anti-Gli3
Assay notes:	Individual E11.5 right hindlimb buds were divided into anterior (A) and posterior (P) halves.

Results

Image: 7

	Sample Information		Bands		Other Sample Information
Lane	Age	Structure	190 kDa (a)	83 kDa (b)	Genetic Background	Mutant Allele(s)	Sex	Note
+/+ A	E11.5	TS19: hindlimb bud	Present	Present	involves: C3H/HeJ * C57BL/6		Not Specified	Anterior
+/+ P	E11.5	TS19: hindlimb bud	Present	Present	involves: C3H/HeJ * C57BL/6		Not Specified	Posterior
Xs^J/+ A	E11.5	TS19: hindlimb bud	Present (c)	Present	involves: C3H/HeJ * C57BL/6	Eif3c^Xs-J/Eif3c⁺	Not Specified	Anterior
Xs^J/+ P	E11.5	TS19: hindlimb bud	Present (d)	Present	involves: C3H/HeJ * C57BL/6	Eif3c^Xs-J/Eif3c⁺	Not Specified	Posterior

Notes:
(a) GLI3A (full length - activator)
(b) GLI3R (processed - repressor)
(c) The ratio of full-length GLI3A to processed GLI3R is dramatically increased in the Eif3c<Xs-J>/+ compared with wild type anterior limb bud.
(d) The ratio of full-length GLI3A to processed GLI3R is similar in the Eif3c<Xs-J>/+ compared with wild type posterior limb bud.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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