Gene Expression Data
Assay Details
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Assay
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Reference:
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J:172775
Gildea DE, et al., The pleiotropic mouse phenotype extra-toes spotting is caused by translation initiation factor Eif3c mutations and is associated with disrupted sonic hedgehog signaling. FASEB J. 2011 May;25(5):1596-605
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7467424
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Gene symbol:
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Ptch1
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Gene name:
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patched 1
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Probe:
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Ptch1 probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
+/+ E10.5:
embryonic day 10.5
(more  )
Specimen
+/+ E10.5:
(close  )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb bud |
Present |
Regionally restricted |
6_Ptch1 E10.5 +/+
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Posterior expression was observed in the limb.
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Specimen
XsJ/+ E10.5:
embryonic day 10.5; Eif3cXs-J/Eif3c +
(more )
Specimen
Xs/+ E10.5:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Mutant Allele(s):
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Eif3cXs-J/Eif3c+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb bud |
Present |
Regionally restricted |
6_Ptch1 E10.5 XsJ/+
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Ectopic anterior expression (1 of 2 embryos) and normal posterior expression was observed in the limb.
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Specimen
+/+ E11.5:
embryonic day 11.5
(more )
Specimen
+/+ E11.5:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb bud |
Present |
Regionally restricted |
6_Ptch1 E11.5 +/+
|
Posterior expression was observed in the limb.
|
Specimen
XsJ/+ E11.5:
embryonic day 11.5; Eif3cXs-J/Eif3c +
(more )
Specimen
Xs/+ E11.5:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Mutant Allele(s):
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Eif3cXs-J/Eif3c+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb bud |
Present |
Regionally restricted |
6_Ptch1 E11.5 XsJ/+
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Ectopic anterior expression (2 of 3 embryos) and normal posterior expression was observed in the limb.
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Specimen
+/+ E12.5:
embryonic day 12.5
(more )
Specimen
+/+ E12.5:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Present |
Regionally restricted |
6_Ptch1 E12.5 +/+
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Posterior expression was observed in the limb.
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Specimen
XsJ/+ E12.5:
embryonic day 12.5; Eif3cXs-J/Eif3c +
(more )
Specimen
Xs/+ E12.5:
(close )
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Genetic Background:
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involves: C3H/HeJ * C57BL/6
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Mutant Allele(s):
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Eif3cXs-J/Eif3c+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Present |
Regionally restricted |
6_Ptch1 E12.5 XsJ/+
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Ectopic anterior expression (3 of 4 embryos) and normal posterior expression was observed in the limb.
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Analysis Tools