Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:172775
Gildea DE, et al., The pleiotropic mouse phenotype extra-toes spotting is caused by translation initiation factor Eif3c mutations and is associated with disrupted sonic hedgehog signaling. FASEB J. 2011 May;25(5):1596-605
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7467421
|
|
Gene symbol:
|
Gli3
|
|
Gene name:
|
GLI-Kruppel family member GLI3
|
|
Probe:
|
Gli3 probe
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
+/+ E10.5:
embryonic day 10.5
(more  )
Specimen
+/+ E10.5:
(close  )
|
Genetic Background:
|
involves: C3H/HeJ * C57BL/6
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb bud |
Present |
|
6_Gli3 E10.5 +/+
|
|
Specimen
XsJ/+ E10.5:
embryonic day 10.5; Eif3cXs-J/Eif3c +
(more )
Specimen
Xs/+ E10.5:
(close )
|
Genetic Background:
|
involves: C3H/HeJ * C57BL/6
|
|
Mutant Allele(s):
|
Eif3cXs-J/Eif3c+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb bud |
Present |
|
6_Gli3 E10.5 XsJ/+
|
Normal expression was observed in the limb.
|
Specimen
+/+ E11.5:
embryonic day 11.5
(more )
Specimen
+/+ E11.5:
(close )
|
Genetic Background:
|
involves: C3H/HeJ * C57BL/6
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb bud |
Present |
|
6_Gli3 E11.5 +/+
|
|
Specimen
XsJ/+ E11.5:
embryonic day 11.5; Eif3cXs-J/Eif3c +
(more )
Specimen
Xs/+ E11.5:
(close )
|
Genetic Background:
|
involves: C3H/HeJ * C57BL/6
|
|
Mutant Allele(s):
|
Eif3cXs-J/Eif3c+
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb bud |
Present |
|
6_Gli3 E11.5 XsJ/+
|
Normal expression was observed in the limb.
|
Specimen
+/+ E12.5:
embryonic day 12.5
(more )
Specimen
+/+ E12.5:
(close )
|
Genetic Background:
|
involves: C3H/HeJ * C57BL/6
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Present |
|
6_Gli3 E12.5 +/+
|
|
Specimen
XsJ/+ E12.5:
embryonic day 12.5; Eif3cXs-J/Eif3c +
(more )
Specimen
Xs/+ E12.5:
(close )
|
Genetic Background:
|
involves: C3H/HeJ * C57BL/6
|
|
Mutant Allele(s):
|
Eif3cXs-J/Eif3c+
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Present |
|
6_Gli3 E12.5 XsJ/+
|
Normal expression was observed in the limb.
|
|
Analysis Tools