Myl7 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:324720 Zhao Q, et al., Drp1 regulates transcription of ribosomal protein genes in embryonic hearts. J Cell Sci. 2022 Feb 15;135(4):jcs258956
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7467167
Gene symbol:	Myl7
Gene name:	myosin, light polypeptide 7, regulatory

Antibody:

anti-MLC2A

Results

Specimen S1 ctrl MLC2A: embryonic day 9.5; Dnm1l^tm1.1Hise/? (more

)
Note: Double labeled: green - MLC2A (Myl7); red - DRP1 (Dnm1l assay MGI:7467166).

Structure	Level	Image
TS15: common atrial chamber	Present	S1_ctrl merge , S1_ctrl MLC2A
TS15: outflow tract	Present	S1_ctrl merge , S1_ctrl MLC2A
TS15: primitive ventricle	Present	S1_ctrl merge , S1_ctrl MLC2A

Specimen S1 mut MLC2A: embryonic day 9.5; Dnm1l^tm1.1Hise/Dnm1l^tm1.1Hise, Tg(Mef2c-cre)2Blk/0 (more

)
Note: Conditional mutant. Double labeled: green - MLC2A (Myl7); red - DRP1 (Dnm1l assay MGI:7467166).

Structure	Level	Image
TS15: common atrial chamber	Present	S1_mut MLC2A , S1_mut merge
TS15: outflow tract	Present	S1_mut MLC2A , S1_mut merge
TS15: primitive ventricle	Present	S1_mut MLC2A , S1_mut merge

Specimen 1A Ctrl (left) MLC2A: embryonic day 10.5; Dnm1l^tm1.1Hise/? (more

)
Note: Double labeled: green - MLC2A (Myl7); red - DRP1 (Dnm1l assay MGI:7467166).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		1A Ctrl (left) MLC2A , 1A Ctrl (left) merge

Specimen 1A Mut (left) MLC2A: embryonic day 10.5; Dnm1l^tm1.1Hise/Dnm1l^tm1.1Hise, Tg(Mef2c-cre)2Blk/0 (more

)
Note: Conditional mutant. Double labeled: green - MLC2A (Myl7); red - DRP1 (Dnm1l assay MGI:7467166).

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present		1A Mut (left) merge , 1A Mut (left) MLC2A

Specimen 1A Ctrl (right) MLC2A: embryonic day 10.5; Dnm1l^tm1.1Hise/? (more

)
Note: Double labeled: green - MLC2A (Myl7); red - DRP1 (Dnm1l assay MGI:7467166).

Structure	Level	Image
TS17: atrioventricular canal	Present	1A Ctrl (right) merge , 1A Ctrl (right ) MLC2A
TS17: common atrial chamber	Present	1A Ctrl (right) merge , 1A Ctrl (right ) MLC2A
TS17: primitive ventricle	Present	1A Ctrl (right) merge , 1A Ctrl (right ) MLC2A

Specimen 1A Mut (right) MLC2A: embryonic day 10.5; Dnm1l^tm1.1Hise/Dnm1l^tm1.1Hise, Tg(Mef2c-cre)2Blk/0 (more

)
Note: Conditional mutant. Double labeled: green - MLC2A (Myl7); red - DRP1 (Dnm1l assay MGI:7467166).

Structure	Level	Image
TS17: atrioventricular canal	Present	1A Mut (right) MLC2A , 1A Mut (right) merge
TS17: common atrial chamber	Present	1A Mut (right) MLC2A , 1A Mut (right) merge
TS17: primitive ventricle	Present	1A Mut (right) MLC2A , 1A Mut (right) merge

Specimen 3A Ctrl MLC2A: embryonic day 12.5; Dnm1l^tm1.1Hise/? (more

)
Note: Double labeled: green - MLC2A (Myl7); red - N-CAD (Cdh2 assay MGI:7467169).

Structure	Level	Pattern	Image	Note
TS20: heart	Present		3A Ctrl MLC2A

Specimen 3A Mut MLC2A: embryonic day 12.5; Dnm1l^tm1.1Hise/? (more

)
Note: Double labeled: green - MLC2A (Myl7); red - N-CAD (Cdh2 assay MGI:7467169).

Structure	Level	Pattern	Image	Note
TS20: heart	Present		3A Mut MLC2A

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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