Nes Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:127447 Kwiatkowski AV, et al., Ena/VASP Is Required for neuritogenesis in the developing cortex. Neuron. 2007 Nov 8;56(3):441-55
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7466761
Gene symbol:	Nes
Gene name:	nestin

Antibody:

anti-Nestin

Results

Specimen 2J: embryonic day 16.5 (more

)
Note: Double labeled with laminin (green). Controls had either one allele of Enah^tm1Fbg and a second Vasp^tm1Ref or Vasp^tm1Ref allele were used as controls.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: cerebral cortex	radial glial cell	Present	Regionally restricted	2J	Expression is in radial glia processes that were organized in a parallel array with tufted endfeet terminating at the pial membrane.

Specimen 2K: embryonic day 16.5; Enah^tm1Fbg/Enah⁺, Evl^tm1Fbg/Evl^tm1Fbg, Vasp^tm1Ref/Vasp^tm1Ref (more

)
Note: Double labeled with laminin (red).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: cerebral cortex	neuron	Present	Regionally restricted	2K	In this mutant expression in the radial glia processes endfeet lacked tufts and appeared detached from the pial membrane.

Specimen 2L: embryonic day 16.5; Enah^tm1Fbg/Enah⁺, Evl^tm1Fbg/Evl^tm1Fbg, Vasp^tm1Ref/Vasp^tm1Ref (more

)
Note: Double labeled with laminin (red).

Structure	Cell Type	Level	Pattern	Image	Note
TS24: cerebral cortex	radial glial cell	Present	Regionally restricted	2L	This mutant has expression in ectopias where the pial membrane was disrupted and radial glial processes extended into ectopias.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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