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Gene Expression Data
Assay Details
Assay
Reference: J:189072 Spiller CM, et al., Endogenous Nodal signaling regulates germ cell potency during mammalian testis development. Development. 2012 Nov;139(22):4123-32
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7466229
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: Anti-DDX4/MVH (ab13840)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 3C +/+: embryonic day 13.5 (more )
Note: Also stained for BrdU (green).
Structure Level Pattern Image Note
TS21: germ cell of testis Present 3E +/+ 27.8% of wild type germ cells are proliferating.
Specimen 3C fl/fl: embryonic day 13.5; Nodaltm1Mku/Nodaltm1Mku (more )
Note: Also stained for BrdU (green).
Structure Level Pattern Image Note
TS21: germ cell of testis Present 3E fl/fl 22.3% of mutant germ cells are proliferating, a reduction compared to rates seen in wild type.
Specimen S3A 11.5 +/+: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present S3A 11.5 +/+
Specimen S3A 11.5 fl/fl: embryonic day 11.5; Nodaltm1Mku/Nodaltm1Mku (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Present S3A 11.5 fl/fl The number of germ cells in the mutant is comparable to that seen in wild type.
Specimen 13.5 +/+: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: germ cell of testis Present
Specimen 13.5 fl/fl: embryonic day 13.5; Nodaltm1Mku/Nodaltm1Mku (more )
Structure Level Pattern Image Note
TS21: germ cell of testis Present The number of germ cells in the mutant is comparable to that seen in wild type.
Specimen S3A 14.5 +/+: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: germ cell of testis Present S3A 14.5 +/+
Specimen S3A 14.5 fl/fl: embryonic day 14.5; Nodaltm1Mku/Nodaltm1Mku (more )
Structure Level Pattern Image Note
TS22: germ cell of testis Present S3A 14.5 fl/fl The number of germ cells in the mutant is comparable to that seen in wild type.
Specimen S3A 17.5 +/+: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: germ cell of testis Present S3A 17.5 +/+
Specimen S3A 17.5 fl/fl: embryonic day 17.5; Nodaltm1Mku/Nodaltm1Mku (more )
Structure Level Pattern Image Note
TS25: germ cell of testis Present S3A 17.5 fl/fl The number of germ cells in the mutant is comparable to that seen in wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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