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Gene Expression Data
Assay Details
Assay
Reference: J:325355 Rong Y, et al., USP16-mediated histone H2A lysine-119 deubiquitination during oocyte maturation is a prerequisite for zygotic genome activation. Nucleic Acids Res. 2022 Jun 10;50(10):5599-5616
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7465076
Gene symbol: Usp16
Gene name: ubiquitin specific peptidase 16
Antibody: anti-USP16 [EPR20914-47] (ab236628)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 2E GV: postnatal adult (more )
Note: Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS28: primary oocyte Present Regionally restricted 2E GV USP16 , 2E GV (merge) Mainly localized in cytoplasm.
Specimen 2E GVBD: postnatal adult (more )
Note: Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS28: secondary oocyte Present Uniform 2E GVBD USP16 , 2E GVBD (merge) After GVBD, expression distributed evenly in ooplasm.
Specimen 2E MI: postnatal adult (more )
Note: Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS28: secondary oocyte Present 2E MI USP16 , 2E MI (merge) Expression remained stable during meiotic maturation.
Specimen 2E MII: postnatal adult (more )
Note: Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS28: secondary oocyte Present 2E MII USP16 , 2E MII (merge) Expression remained stable during meiotic maturation.
Specimen 2E Zygote: embryonic day 0.5 (more )
Note: Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present Regionally restricted 2E Zygote USP16 , 2E Zygote (merge) Expression continued to accumulate during early embryo development. Mainly localized in cytoplasm of early embryos
Specimen 2E 2-cell: embryonic day 1.0 (more )
Note: Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present Regionally restricted 2E 2-cell USP16 , 2E 2-cell (merge) Expression continued to accumulate during early embryo development. Mainly localized in cytoplasm of early embryos
Specimen 2E 4-cell: embryonic day 2.0 (more )
Note: Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present Regionally restricted 2E 4-cell (merge) , 2E 4-cell USP16 Expression continued to accumulate during early embryo development. Mainly localized in cytoplasm of early embryos
Specimen 2E 8-cell: embryonic day 2.5 (more )
Note: Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Strong Regionally restricted 2E 8-cell USP16 , 2E 8-cell (merge)
Specimen 2E Morula: embryonic day 3.0 (more )
Note: Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS4: compacted morula Very strong Regionally restricted 2E Morula (merge) , 2E Morula USP16 Expression continued to accumulate during early embryo development. Mainly localized in cytoplasm of early embryos
Specimen 2E Blastocyst: embryonic day 3.5 (more )
Note: Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS4: blastocyst Strong Regionally restricted 2E Blastocyst USP16 , 2E Blastocyst (merge) Expression continued to accumulate during early embryo development. Mainly localized in cytoplasm of early embryos
Specimen S4C WT: postnatal adult (more )
Structure Level Pattern Image Note
TS28: oocyte Present Regionally restricted S4C WT (upper) , S4C WT (lower) Mainly localized in cytoplasm.
Specimen S4C fl/fl;Cre: postnatal adult; Tg(Gdf9-icre)5092Coo/0, Usp16tm2.1Hbwg/Usp16tm2.1Hbwg (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS28: oocyte Absent S4C fl/fl;Cre (upper) , S4C fl/fl;Cre (lower) Efficient protein depletion confirmed.
Specimen 7F 4-cell WT: embryonic day 2.0 (more )
Note: Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Present 7F 4-cell WT USP16 , 7F 4-cell WT (merge)
Specimen 7F 4-cell +/- (left): embryonic day 2.0; Tg(Gdf9-icre)5092Coo/0, Usp16tm2.1Hbwg/Usp16+ (more )
Note: Conditional mutant. Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent 7F 4-cell Usp16-/+ USP16 (left) , 7F 4-cell Usp16-/+ (left merge) Expression undetectable at 4-cell stage.
Specimen 7F 4-cell +/- (right): embryonic day 2.0; Tg(Gdf9-icre)5092Coo/0, Usp16tm2.1Hbwg/Usp16+ (more )
Note: Conditional mutant. Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent 7F 4-cell Usp16-/+ USP16 (right) , 7F 4-cell Usp16-/+ (right merge) Expression undetectable at 4-cell stage.
Specimen 7F blastocyst WT: embryonic day 3.5 (more )
Note: Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS4: blastocyst Present 7F blastocyst WT USP16 , 7F blastocyst WT (merge)
Specimen 7F blastocyst -/+: embryonic day 3.5; Tg(Gdf9-icre)5092Coo/0, Usp16tm2.1Hbwg/Usp16+ (more )
Note: Conditional mutant. Double labeled: red - Usp16; green - alpha-tubulin.
Structure Level Pattern Image Note
TS4: blastocyst Present 7F blastocyst Usp16-/+ USP16 , 7F blastocyst Usp16-/+ (merge) Expression present in embryos that develop into blastocysts.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory