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Gene Expression Data
Assay Details
Assay
Reference: J:325355 Rong Y, et al., USP16-mediated histone H2A lysine-119 deubiquitination during oocyte maturation is a prerequisite for zygotic genome activation. Nucleic Acids Res. 2022 Jun 10;50(10):5599-5616
Assay type: RT-PCR
MGI Accession ID: MGI:7465040
Gene symbol: Usp16
Gene name: ubiquitin specific peptidase 16
Probe: Usp16-pF, Usp16-pR
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh.
Results
Image: 2B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
GV P adult TS28: primary oocyte Present (a) Not Specified involves: C57BL/6 Female
MII P adult TS28: secondary oocyte Present (a) Not Specified involves: C57BL/6 Female
Zygote E0.5 (b) TS1: 1-cell stage embryo Weak (a) Not Specified involves: C57BL/6 Not Specified
2-cell E1.0 (b) TS2: 2-cell stage embryo Very strong (a) Not Specified involves: C57BL/6 Not Specified
4-cell E2.0 (b) TS3: 4-cell stage embryo Strong (a) Not Specified involves: C57BL/6 Not Specified
8-cell E2.5 (b) TS3: 8-cell stage embryo Strong (a) Not Specified involves: C57BL/6 Not Specified
Morula E3.0 (b) TS4: compacted morula Trace Not Specified involves: C57BL/6 Not Specified
Blastocyst E3.5 (b) TS4: blastocyst Trace Not Specified involves: C57BL/6 Not Specified
Heart P adult TS28: heart Trace Not Specified involves: C57BL/6 Not Specified
Liver P adult TS28: liver Trace Not Specified involves: C57BL/6 Not Specified
Spleen P adult TS28: spleen Trace Not Specified involves: C57BL/6 Not Specified
Lung P adult TS28: lung Trace Not Specified involves: C57BL/6 Not Specified
Kidney P adult TS28: metanephros Trace Not Specified involves: C57BL/6 Not Specified
Ovary P adult TS28: ovary Trace Not Specified involves: C57BL/6 Female
Testis P adult TS28: testis Trace Not Specified involves: C57BL/6 Male
Brain P adult TS28: brain Trace Not Specified involves: C57BL/6 Not Specified
Notes:
(a) Significantly higher expression in oocytes and early embryos than in other tissues.
(b) Age assigned by curator based on morphological criteria supplied by authors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory