Acta2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:226036 Maddala R, et al., Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis. Dev Biol. 2015 Oct 1;406(1):74-91
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7464765
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:	anti-smooth muscle alpha actin
Detection system:	Cy3

Results

7A E13.5 WT ©

Specimen 7A E13.5 WT: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: lens epithelium	Absent		7A E13.5 WT
TS21: lens fiber	Absent		7A E13.5 WT

7A E13.5 cKO ©

Specimen 7A E13.5 cKO: embryonic day 13.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS21: lens epithelium	Weak		7A E13.5 cKO
TS21: lens fiber	Absent		7A E13.5 cKO

7A E15.5 WT ©

Specimen 7A E15.5 WT: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: lens epithelium	Absent		7A E15.5 WT
TS23: lens fiber	Absent		7A E15.5 WT
TS23: eye anterior segment	Present	Regionally restricted	7A E15.5 WT	Expressed in the presumptive ciliary body and iris.

7A E15.5 cKO ©

Specimen 7A E15.5 cKO: embryonic day 15.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: lens epithelium	Present		7A E15.5 cKO	Progressively increased level of expression was seen.
TS23: lens fiber	Absent		7A E15.5 cKO
TS23: eye anterior segment	Present	Regionally restricted	7A E15.5 cKO	Expressed in the presumptive ciliary body and iris.

7A E17.5 WT ©

Specimen 7A E17.5 WT: embryonic day 17.5 (more

)

Structure	Level	Image
TS25: ciliary body	Present	7A E17.5 WT
TS25: iris	Present	7A E17.5 WT
TS25: lens epithelium	Absent	7A E17.5 WT
TS25: lens fiber	Absent	7A E17.5 WT

7A E17.5 cKO ©

Specimen 7A E17.5 cKO: embryonic day 17.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Image	Note
TS25: lens epithelium	Strong	7A E17.5 cKO	Progressively increased level of expression was seen, localized intensely to the cell-cell junctions.
TS25: lens fiber	Absent	7A E17.5 cKO
TS25: ciliary body	Present	7A E17.5 cKO
TS25: iris	Present	7A E17.5 cKO

Specimen not shown: embryonic day 15.5; Tg(Pax6-cre,GFP)1Pgr/0 (more

)

Structure	Level	Pattern	Image	Note
TS23: lens epithelium	Absent
TS23: lens fiber	Absent

Specimen not shown: embryonic day 17.5; Tg(Pax6-cre,GFP)1Pgr/0 (more

)

Structure	Level	Pattern	Image	Note
TS25: lens epithelium	Absent
TS25: lens fiber	Absent

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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