Itgb1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:226036 Maddala R, et al., Rap1 GTPase is required for mouse lens epithelial maintenance and morphogenesis. Dev Biol. 2015 Oct 1;406(1):74-91
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7464653
Gene symbol:	Itgb1
Gene name:	integrin beta 1 (fibronectin receptor beta)

Antibody:	9EG7
Detection system:	Secondary antibody coupled to Alexa Fluor 594
Assay notes:	The antibody used recognizes the activated form of the protein.

Results

6B WT ©

Specimen 6B WT: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: lens epithelium	Weak	Widespread	6B WT
TS25: lens fiber	Weak	Widespread	6B WT

6B cKO ©

Specimen 6B cKO: embryonic day 17.5; Rap1a^tm1Morz/Rap1a^tm1Morz, Rap1b^tm1Morz/Rap1b^tm1Morz, Tg(Pax6-cre,GFP)1Pgr/0 (more

)
Note: Conditional mutant.

Structure	Level	Image	Note
TS25: lens anterior epithelium	Strong	6B cKO	A more intense and robust increase in expression relative to the equatorial epithelium, compared with the control.
TS25: lens fiber	Weak	6B cKO	Some decrease in the staining compared with the control.
TS25: lens equatorial epithelium	Present	6B cKO	A robust increase in expression compared with the control.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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