Antibody:	anti-p-Y118-Paxillin
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS23: lens epithelium	Strong		6A E15.5 WT lower , 6A E15.5 WT upper	Expressed discretely and intensely at the apical junctions of primary lens fibers and epithelial cells.
TS23: lens capsule	Present	Widespread	6A E15.5 WT upper , 6A E15.5 WT lower	There was also some staining throughout the lens capsule.

Structure	Level	Pattern	Image	Note
TS23: lens epithelium	Trace		6A E15.5 cKO upper , 6A E15.5 cKO lower	There was an almost complete loss of staining at the apical junctions of epithelial and fiber cells.

Structure	Level	Pattern	Image	Note
TS25: lens epithelium	Strong		6A E17.5 WT upper , 6A E17.5 WT lower	Expressed discretely and intensely at the apical junctions of primary lens fibers and epithelial cells.
TS25: lens capsule	Present	Widespread	6A E17.5 WT lower , 6A E17.5 WT upper	There was also some staining throughout the lens capsule.

Structure	Level	Pattern	Image	Note
TS25: lens epithelium	Trace		6A E17.5 cKO upper , 6A E17.5 cKO lower	There was an almost complete loss of staining at the apical junctions of epithelial and fiber cells.