Gene Expression Data
Assay Details
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Assay
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Reference:
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J:173287
Long JE, et al., Dlx1&2 and Mash1 transcription factors control striatal patterning and differentiation through parallel and overlapping pathways. J Comp Neurol. 2009 Feb 1;512(4):556-72
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7464519
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Gene symbol:
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Tac1
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Gene name:
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tachykinin 1
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Probe:
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IMAGE clone 1166182
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Table 4 data was incorporated into Fig. 7 & S2 samples, as per the text.
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Results
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Specimen
6j:
embryonic day 15.5
(more  )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
6j:
(close  )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The genetic background of the specimen
was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: corpus striatum |
Present |
Regionally restricted |
6j
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Expression marks the striatal differentiated state.
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Specimen
6j':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: corpus striatum |
Weak |
Regionally restricted |
6j'
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Expression was decreased in the striatum. Residual expression was seen in superficial parts of the striatal mantle.
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Specimen
7k:
embryonic day 15.5
(more )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
7k:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The genetic background of the specimen
was either wild type or heterozygous.
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Specimen
7k':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Weak |
Regionally restricted |
7k'
,
S2h'
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Expression was severely decreased in the mantle zone. It was undetectable in the ventricular zone and subventricular zone.
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Specimen
7k'':
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Weak |
Regionally restricted |
7k''
,
S2h''
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Expression was severely decreased in the ventral mantle zone (unchanged in dorsal MZ). It was undetectable in the ventricular zone and subventricular zone.
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Specimen
7k''':
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Weak |
Regionally restricted |
7k'''
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Expression was severely decreased in the mantle zone. It was undetectable in the ventricular zone and subventricular zone.
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Analysis Tools