Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:173287
Long JE, et al., Dlx1&2 and Mash1 transcription factors control striatal patterning and differentiation through parallel and overlapping pathways. J Comp Neurol. 2009 Feb 1;512(4):556-72
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7464514
|
|
Gene symbol:
|
Gad1
|
|
Gene name:
|
glutamate decarboxylase 1
|
|
Probe:
|
Gad67 cDNA
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
Table 4 data was incorporated into Fig. 7 & S2 samples, as per the text.
|
|
|
Results
|
Specimen
6g:
embryonic day 15.5
(more  )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
6g:
(close  )
|
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6J
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
The genetic background of the specimen
was either wild type or heterozygous.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: corpus striatum |
Present |
Regionally restricted |
6g
|
Expression marks the striatal differentiated state.
|
Specimen
6g':
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: corpus striatum |
Weak |
Regionally restricted |
6g'
|
Expression was decreased in the striatum. Residual expression was seen in superficial parts of the striatal mantle.
|
Specimen
7l:
embryonic day 15.5
(more )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
7l:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
The genetic background of the specimen
was either wild type or heterozygous.
|
Specimen
7l':
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Weak |
Regionally restricted |
7l'
,
S2e'
|
Expression was severely decreased in the subventricular zone and mantle zone. It was undetectable in the ventricular zone.
|
| TS23: telencephalon septum |
Present |
Regionally restricted |
7l'
,
S2e'
|
Expression was unchanged in the subventricular zone and mantle zone. It was undetectable in the ventricular zone.
|
Specimen
7l'':
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6J
|
|
Mutant Allele(s):
|
Ascl1tm1And/Ascl1tm1And
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Regionally restricted |
S2e''
,
7l''
|
Expression was increased in the subventricular zone and unchanged in the mantle zone. It was undetectable in the ventricular zone.
|
| TS23: telencephalon septum |
Present |
Regionally restricted |
S2e''
,
7l''
|
Expression was unchanged in the dorsal subventricular zone and mantle zone. Expression was moderately/mildly decreased in the ventral subventricular zone and mantle zone. It was undetectable in the ventricular zone.
|
Specimen
7l''':
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Weak |
Regionally restricted |
7l'''
|
Expression was severely decreased in the subventricular zone and mantle zone. It was undetectable in the ventricular zone.
|
| TS23: telencephalon septum |
Weak |
Regionally restricted |
7l'''
|
Expression was severely decreased in the subventricular zone and mantle zone. It was undetectable in the ventricular zone.
|
Specimen
S1g:
embryonic day 12.5
(more )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
S1g:
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6J
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
The genetic background of the specimen
was either wild type or heterozygous.
|
Specimen
S1g':
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Analysis Tools