Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:173287
Long JE, et al., Dlx1&2 and Mash1 transcription factors control striatal patterning and differentiation through parallel and overlapping pathways. J Comp Neurol. 2009 Feb 1;512(4):556-72
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7464496
|
|
Gene symbol:
|
Isl1
|
|
Gene name:
|
ISL1 transcription factor, LIM/homeodomain
|
|
Probe:
|
Islet1 cDNA
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
Table 4 data was incorporated into Fig. 7 & S2 samples, as per the text.
|
|
|
Results
|
Specimen
5o:
embryonic day 15.5
(more  )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
5o:
(close  )
|
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6J
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
The genetic background of the specimen
was either wild type or heterozygous.
|
Specimen
5o':
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
7d:
embryonic day 15.5
(more )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
7d:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
The genetic background of the specimen
was either wild type or heterozygous.
|
Specimen
7d':
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Regionally restricted |
7d'
|
Expression was moderately/mildly decreased in the subventricular zone and mantle zone. It was undetectable in the ventricular zone.
|
| TS23: telencephalon septum |
Present |
Regionally restricted |
7d'
|
Expression was increased in the mantle zone. Expression was severely decreased in the subventricular zone and undetectable in the ventricular zone.
|
Specimen
7d'':
(close )
|
Genetic Background:
|
involves: 129 * C57BL/6J
|
|
Mutant Allele(s):
|
Ascl1tm1And/Ascl1tm1And
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Regionally restricted |
7d''
,
S2j''
|
Expression was increased in the dorsal subventricular zone and severely decreased in the ventral subventricular zone and ventral mantle zone. It was undetectable in the ventricular zone.
|
| TS23: telencephalon septum |
Weak |
Regionally restricted |
7d''
,
S2j''
|
Expression was severely decreased in the subventricular zone and mantle zone. It was undetectable in the ventricular zone.
|
Specimen
7d''':
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Weak |
Regionally restricted |
7d'''
|
Expression was increased in the mantle zone and unchanged in the subventricular zone. It was undetectable in the ventricular zone.
|
| TS23: telencephalon septum |
Weak |
Regionally restricted |
7d'''
|
Expression was severely decreased in the subventricular zone and mantle zone. It was undetectable in the ventricular zone.
|
Specimen
S2j':
(close )
|
Genetic Background:
|
involves: 129X1/SvJ * C57BL/6J
|
|
Mutant Allele(s):
|
Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Regionally restricted |
S2j'
|
Expression was moderately/mildly decreased in the subventricular zone and mantle zone. It was undetectable in the ventricular zone.
|
| TS23: telencephalon septum |
Present |
Regionally restricted |
S2j'
|
Expression was increased in the mantle zone. Expression was severely decreased in the subventricular zone and undetectable in the ventricular zone.
|
|
Analysis Tools