Gene Expression Data
Assay Details
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Assay
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Reference:
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J:173287
Long JE, et al., Dlx1&2 and Mash1 transcription factors control striatal patterning and differentiation through parallel and overlapping pathways. J Comp Neurol. 2009 Feb 1;512(4):556-72
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7464471
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Gene symbol:
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Ascl1
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Gene name:
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achaete-scute family bHLH transcription factor 1
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Probe:
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Mash1 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Table 4 data was incorporated into Fig. 7 &S2 samples, as per the text.
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Results
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Specimen
3k:
embryonic day 15.5
(more  )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
3k:
(close  )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The genetic background of the specimen
was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Regionally restricted |
3k
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Expression was detected in the ventricular zone and/or subventricular zone.
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Specimen
3k':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Regionally restricted |
3k'
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Expression was upregulated in the lateral ganglionic eminence, particularly in the subventricular zone.
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Specimen
7a:
embryonic day 15.5
(more )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
7a:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The genetic background of the specimen
was either wild type or heterozygous.
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Specimen
7a':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Regionally restricted |
S2b'
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7a'
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Expression was increased in the ventricular zone and subventricular zone. It was undetectable in the mantle zone.
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| TS23: telencephalon septum |
Present |
Regionally restricted |
S2b'
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7a'
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Expression was unchanged in the ventricular zone. It was undetectable in the subventricular zone and mantle zone.
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Specimen
7a'':
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Regionally restricted |
7a''
,
S2b''
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Expression was unchanged in the ventricular zone and undetectable in the subventricular zone and mantle zone. Supplemental Table 2 indicates no major change.
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| TS23: telencephalon septum |
Present |
Regionally restricted |
S2b''
,
7a''
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Expression was severely decreased in the ventricular zone and undetectable in the subventricular zone and mantle zone.
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Specimen
7a''':
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Regionally restricted |
7a'''
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Expression was increased in the subventricular zone. It was unchanged in the ventricular zone and undetectable in the mantle zone. Supplemental Table 2 indicates no major change.
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| TS23: telencephalon septum |
Present |
Regionally restricted |
7a'''
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Expression was unchanged in the ventricular zone and undetectable in the subventricular zone and mantle zone.
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Specimen
S1n:
embryonic day 12.5
(more )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
S1n:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The genetic background of the specimen
was either wild type or heterozygous.
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Specimen
S1n':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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