Gene Expression Data
Assay Details
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Assay
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Reference:
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J:173287
Long JE, et al., Dlx1&2 and Mash1 transcription factors control striatal patterning and differentiation through parallel and overlapping pathways. J Comp Neurol. 2009 Feb 1;512(4):556-72
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7464420
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Gene symbol:
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Dlx1
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Gene name:
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distal-less homeobox 1
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Probe:
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Dlx1 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The probe recognized the truncated transcript in the mutant. Table 4 data was incorporated into Fig. 7 &S2 samples, as per the text.
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Results
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Specimen
1a:
embryonic day 15.5
(more  )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
1a:
(close  )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The genetic background of the specimen
was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Graded |
1a
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Expressed in a dorsoventral grandient in progenitor cells of the lateral ganglionic eminence. Expression was particularly high in the dorsal LGE, where it was detected in most cells in both the ventricular and subventricular zones.
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| TS23: telencephalon septum |
Present |
Graded |
1a
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Expressed in a dorsoventral grandient.
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Specimen
1a':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Weak |
Regionally restricted |
1a'
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Expression continued at low levels through the subventricular zone. Expression in the mantle zone was not detectable.
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Specimen
7e:
embryonic day 15.5
(more )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
7e:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The genetic background of the specimen
was either wild type or heterozygous.
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Specimen
7e':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Regionally restricted |
7e'
,
S2a'
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Expression was moderately/mildly decreased in the ventricular zone and subventricular zone. Expression was severely decreased in the mantle zone.
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| TS23: telencephalon septum |
Weak |
Regionally restricted |
S2a'
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7e'
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Expression was moderately/mildly decreased in the subventricular zone. Expression was severely decreased in the mantle zone. It was undetectable in the ventricular zone.
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Specimen
7e'':
(close )
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Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Ascl1tm1And/Ascl1tm1And
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Regionally restricted |
7e''
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S2a''
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Expression was increased in the ventricular zone and subventricular zone. Expression was unchanged in the mantle zone.
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| TS23: telencephalon septum |
Present |
Regionally restricted |
S2a''
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7e''
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Expression was increased in the subventricular zone. Expression was unchanged in the mantle zone. It was undetectable in the ventricular zone.
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Specimen
7e''':
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lateral ganglionic eminence |
Present |
Regionally restricted |
7e'''
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Expression was severely decreased in the subventricular zone and mantle zone. Expression was unchanged in the ventricular zone.
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| TS23: telencephalon septum |
Weak |
Regionally restricted |
7e'''
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Expression was severely decreased in the subventricular zone and mantle zone. Expression was unchanged in the ventricular zone.
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Specimen
S1a:
embryonic day 12.5
(more )
Note: The genetic background of the specimen
was either wild type or heterozygous.
Specimen
S1a:
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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The genetic background of the specimen
was either wild type or heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lateral ganglionic eminence |
Present |
Graded |
S1a
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Expressed in a dorsoventral grandient in progenitor cells of the lateral ganglionic eminence.
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Specimen
S1a':
(close )
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Genetic Background:
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involves: 129X1/SvJ * C57BL/6J
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Mutant Allele(s):
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Del(2Dlx1-Dlx2)1Jlr/Del(2Dlx1-Dlx2)1Jlr
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: lateral ganglionic eminence |
Weak |
Regionally restricted |
S1a'
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Expression continued at low levels through the subventricular zone. Expression in the mantle zone was not detectable.
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