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Gene Expression Data
Assay Details
Assay
Reference: J:309436 Parish EV, et al., Expression of Barhl2 and its relationship with Pax6 expression in the forebrain of the mouse embryo. BMC Neurosci. 2016 Nov 25;17(1):76
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7461784
Gene symbol: Pax6
Gene name: paired box 6
Antibody: anti-Pax6
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 6A-J: embryonic day 12.5 (more )
Note: Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
Structure Level Pattern Image Note
TS20: forebrain-midbrain boundary region Present Regionally restricted 6A Co-expressed with Barhl2 in the pretectum.
TS20: thalamus Present Graded 6C , 6I , 6E , 6G , 6A Cells closer to the pretectum had higher expression and co-expressed with Barhl2.

Specimen 6K/M: embryonic day 12.5 (more )
Note: Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
Structure Level Pattern Image Note
TS20: forebrain Present Regionally restricted 6M , 6K At the eminentia thalami area, Pax6-expressing and Barhl2-expressing cells are clearly segregated.

Specimen 7A/C: embryonic day 10.5 (more )
Note: Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 7A , 7C In the thalamus, cells closer to the pretectum had higher expression and co-expressed with Barhl2. Expressed in prethalamus.
TS17: forebrain-midbrain boundary region Present Regionally restricted 7A , 7C Expressed in the pretectum.

Specimen 7D/F: embryonic day 11.5 (more )
Note: Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
Structure Level Pattern Image Note
TS19: diencephalon Present Graded 7F , 7D Expressed in the thalamus, cells closer to the pretectum had higher expression and co-expressed with Barhl2.
TS19: prethalamus Present 7F , 7D
TS19: forebrain-midbrain boundary region Present Regionally restricted 7D , 7F Expressed in the pretectum.

Specimen 7G/I: embryonic day 12.5 (more )
Note: Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
Structure Level Pattern Image Note
TS20: thalamus Present Graded 7I , 7G Cells closer to the pretectum had higher expression and co-expressed with Barhl2.
TS20: prethalamus Present 7G , 7I
TS20: forebrain-midbrain boundary region Present Regionally restricted 7G , 7I Expressed in the pretectum.

Specimen 7J/L: embryonic day 13.5 (more )
Note: Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
Structure Level Pattern Image Note
TS21: thalamus Weak 7L , 7J Very low expression.
TS21: prethalamus Present 7J , 7L


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory