Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:55507
French MM, et al., Expression of the heparan sulfate proteoglycan, perlecan, during mouse embryogenesis and perlecan chondrogenic activity in vitro. J Cell Biol. 1999 May 31;145(5):1103-15
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:7461251
|
|
Gene symbol:
|
Hspg2
|
|
Gene name:
|
perlecan (heparan sulfate proteoglycan 2)
|
|
Probe:
|
Pln cDNA (clone 5)
|
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
|
Visualized with:
|
Autoradiography
|
|
Assay notes:
|
Sections were fixed in 4% paraformaldehyde.
|
|
|
Results
|
Specimen
3A:
embryonic day 14.5
(more  )
Specimen
3A:
(close  )
|
Genetic Background:
|
CF-1
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cartilage condensation |
Present |
|
3A
|
Expression accumulated in developing cartilage primoridum and was higher in the discs than in the surrounding tissue.
|
| TS22: intervertebral disc |
Present |
|
3A
|
Expression accumulated in developing cartilage primoridum and was higher in the discs than in the surrounding tissue.
|
Specimen
6E/F:
embryonic day 15.5
(more )
Specimen
6E/F:
(close )
|
Genetic Background:
|
CF-1
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cartilage condensation |
Present |
|
6E/F
|
At E15.5, staining did not show a preferential accumulation of mRNA in cartilage primordia relative to the surrounding tissue.
|
|
Analysis Tools
