Antibody:	anti-Trpv4 (#ab39260)
Assay notes:	Adult eyes were fixed in 0.75% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS28: lens	Strong		4B	Expression extends from the anterior to posterior poles and from the equatorial epithelium to the lens core.
TS28: lens equatorial epithelium	Present		4B

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Strong		5E , 5C	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.
TS28: lens equatorial epithelium	Strong		5E , 5C	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.
TS28: lens fiber	Strong		5E , 5C	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Strong		5G , 5I	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.
TS28: lens equatorial epithelium	Strong		5G , 5I	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.
TS28: lens fiber	Strong		5G , 5I	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Strong		5M , 5K	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.
TS28: lens equatorial epithelium	Strong		5M , 5K	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.
TS28: lens fiber	Strong		5M , 5K	There is punctate labeling of the cytoplasm in cells located at the anterior pole, equator, and posterior pole.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present	Non-Uniform	7B , 7G	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present	Non-Uniform	7B , 7G	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens fiber	Present	Non-Uniform	7B , 7G	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present	Non-Uniform	7C , 7H	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present	Non-Uniform	7C , 7H	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens fiber	Present	Non-Uniform	7C , 7H	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present	Non-Uniform	7I , 7D	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present	Non-Uniform	7I , 7D	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens fiber	Present	Non-Uniform	7I , 7D	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present	Non-Uniform	7E , 7J	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present	Non-Uniform	7E , 7J	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens fiber	Present	Non-Uniform	7E , 7J	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present	Non-Uniform	7K , 7F	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present	Non-Uniform	7K , 7F	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.
TS28: lens fiber	Present	Non-Uniform	7K , 7F	Expression is in all regions of the lens except for a narrow region in the inner cortex where a decrease in signal was detected in the absence of pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present		7R , 7M	Pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present		7R , 7M	Pre-treatment with Triton X-100.
TS28: lens fiber	Present		7R , 7M	Pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present		7S , 7N	Pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present		7S , 7N	Pre-treatment with Triton X-100.
TS28: lens fiber	Present		7S , 7N	Pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present		7T , 7O	Pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present		7T , 7O	Pre-treatment with Triton X-100.
TS28: lens fiber	Present		7T , 7O	Pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present		7P , 7U	Pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present		7P , 7U	Pre-treatment with Triton X-100.
TS28: lens fiber	Present		7P , 7U	Pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS28: lens anterior epithelium	Present		7Q , 7V	Pre-treatment with Triton X-100.
TS28: lens equatorial epithelium	Present		7Q , 7V	Pre-treatment with Triton X-100.
TS28: lens fiber	Present		7Q , 7V	Pre-treatment with Triton X-100.

Structure	Level	Pattern	Image	Note
TS16: lens primordium	Strong		8B	Expression is at the membranes.

Structure	Level	Pattern	Image	Note
TS18: lens vesicle	Present		8D	Expression is at the membranes.

Structure	Level	Pattern	Image	Note
TS26: lens epithelium	Present		8G	Expression is in the cytoplasm in the periphery of the outer cortex and in the membrane in the inner cortex and core of the lens.
TS26: lens fiber	Present		8G	Expression is in the cytoplasm in the periphery of the outer cortex and in the membrane in the inner cortex and core of the lens.

Structure	Level	Pattern	Image	Note
TS26: lens epithelium	Present		8I	Expression is in the cytoplasm in the periphery of the outer cortex and in the membrane in the inner cortex and core of the lens.
TS26: lens fiber	Present		8I	Expression is in the cytoplasm in the periphery of the outer cortex and in the membrane in the inner cortex and core of the lens.

Structure	Level	Pattern	Image	Note
TS26: lens epithelium	Present		8K	Expression is in the cytoplasm in the periphery of the outer cortex and in the membrane in the inner cortex and core of the lens.
TS26: lens fiber	Present		8K	Expression is in the cytoplasm in the periphery of the outer cortex and in the membrane in the inner cortex and core of the lens.