Antibody:	anti-ephrinA533
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS24: neocortex	Strong	Regionally restricted	1_wt	Strong expression especially in parietal cortex.

Structure	Level	Pattern	Image	Note
TS24: brain	Absent		1_eA5KO

Structure	Level	Pattern	Image	Note
TS20: neocortex	Strong		2a	Well-stained cell profiles observed in neocortex.

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Strong	Graded	2b	Lateral and ventral (high) to medial and dorsal (low) gradient of expression in lateral ganglionic eminence differentiating zone.

Structure	Level	Pattern	Image	Note
TS20: medial ganglionic eminence	Strong	Graded	2c	Lateral and ventral (high) to medial and dorsal (low) gradient of expression in medial ganglionic eminence differentiating zone.

Structure	Level	Pattern	Image	Note
TS20: preoptic area	Strong	Graded	2d	Lateral and ventral (high) to medial and dorsal (low) gradient of expression in preoptic area differentiating zone.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Strong		2e

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Strong		2f	Strong expression in ventral thalamus.

Structure	Level	Pattern	Image	Note
TS20: metencephalon	Moderate		2g
TS20: midbrain-hindbrain junction	Present		2g	Expression in isthmus.
TS20: cerebellum primordium	Present		2g

Structure	Level	Pattern	Image	Note
TS20: neocortex	Strong	Regionally restricted	2h	Well-stained cell profiles observed in piriform cortex.
TS20: olfactory nerve	Strong		2h
TS20: preoptic area	Strong		2h

Structure	Level	Pattern	Image	Note
TS20: lateral ganglionic eminence	Strong	Regionally restricted	2i	Strong expression in lateral ganglionic eminence differentiating zone.

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Strong	Graded	2j

Structure	Level	Pattern	Image	Note
TS20: midbrain	Strong		2k	Strong expression in mesencephalic tegmentum. No expression in ventral mesencephalon.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Strong	Graded	2l	Ventral and rostral (high) to dorsal and caudal (low) gradient of expression in hypothalamus.

Structure	Level	Pattern	Image	Note
TS22: main olfactory bulb	Moderate		3a

Structure	Level	Pattern	Image	Note
TS22: cortical plate	Strong	Regionally restricted	3b	Strongly expressed in neocortex, especially in cortical plate of primitive somatosensory cortex.

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence	Strong	Graded	3c	Lateral and ventral (high) to medial and dorsal (low) gradient of expression in lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence	Strong		3d
TS22: prethalamus	Strong		3d	Strong expression in ventral thalamus.

Structure	Level	Pattern	Image	Note
TS22: telencephalon subventricular zone	Moderate		3e

Structure	Level	Pattern	Image	Note
TS22: piriform cortex	Strong		3f
TS22: lateral ganglionic eminence	Strong		3f

Structure	Level	Pattern	Image	Note
TS22: pretectum	Strong	Graded	3g	Lateral and ventral (high) to medial and dorsa (low) gradient of expression in pretectum.
TS22: superior colliculus	Strong	Regionally restricted	3g	Strong expression in commissure of superior colliculus.

Structure	Level	Pattern	Image	Note
TS22: 3rd ventricle ependyma	Strong		3h	Strong expression in neuroepithelium of 3rd ventricle.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Strong	Regionally restricted	3i	Strongly expressed in subthalamic area.
TS22: 3rd ventricle ependyma	Strong		3i	Strong expression in neuroepithelijm of 3rd ventricle.

Structure	Level	Pattern	Image	Note
TS22: midbrain tegmentum	Strong		3j	Strong expression in mesencephalic tegmentum

Structure	Level	Pattern	Image	Note
TS22: inferior colliculus	Strong	Graded	3k	Lateral (high) to medial (low) gradient of expression in inferior colliculus.
TS22: superior colliculus	Strong	Regionally restricted	3k	Strong expression in commissure of superior colliculus.

Structure	Level	Pattern	Image	Note
TS22: midbrain-hindbrain junction	Weak	Regionally restricted	3l	Weak expression in isthmus differentiating zone.

Structure	Level	Pattern	Image	Note
TS22: 4th ventricle ependyma	Strong		3m	Strong expression in neuroepithelium of 4th ventricle.

Structure	Level	Pattern	Image	Note
TS22: lateral ganglionic eminence	Strong	Graded	3n	Ventral and rostral (high) to dorsal and caudal (low) gradienct of expression in lateral ganglionic eminence.
TS22: preoptic area	Strong		3n
TS22: prethalamus	Strong	Graded	3n	Ventral and rostral (high) to dorsal and caudal (low) gradienct of expression in thalamus.

Structure	Level	Pattern	Image	Note
TS22: midbrain tegmentum	Strong		3o	Strong expression in mesencephalic tegmentum.
TS22: midbrain	Present	Regionally restricted	3o	No expression in ventral mesencephalon.
TS22: cerebral aqueduct	Strong		3o

Structure	Level	Pattern	Image	Note
TS22: cerebellum	Strong	Regionally restricted	3p	Strong expression in cerebellar vermis.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Moderate		3q

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb prospective granule cell layer	Strong		4a

Structure	Level	Pattern	Image	Note
TS24: main olfactory bulb mitral cell layer	Present		4b
TS24: neocortex	Strong	Regionally restricted	4b	Strong expresion in frontal cortex.

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Strong		4c
TS24: cerebral cortex marginal layer	Strong	Regionally restricted	4c	Strong expression in marginal zone of primitive somatosensory cortex.
TS24: cortical plate	Strong		4c
TS24: cortical subplate	Strong		4c

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Strong		4d
TS24: neocortex	Strong	Regionally restricted	4d	Strong expression in parietal cortex. Less intense expression in insular cortex.

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Strong	Graded	4e	Ventral and rostral (high) to dorsal and caudal (low) gradient of expression.
TS24: olfactory cortex	Strong	Regionally restricted	4e	Strong expression in olfactory tubercle.

Structure	Level	Pattern	Image	Note
TS24: retrosplenial cortex	Moderate		4f

Structure	Level	Pattern	Image	Note
TS24: thalamus	Strong		4g

Structure	Level	Pattern	Image	Note
TS24: piriform cortex	Moderate		4h
TS24: olfactory tract	Strong	Regionally restricted	4h	Strong expression in lateral olfactory tract.

Structure	Level	Pattern	Image	Note
TS24: dorsal thalamus	Present	Graded	4i	Ventral and rostral (high) to dorsal and caudal (low) gradient of expression in thalamus.
TS24: prethalamus	Present	Graded	4i	Ventral and rostral (high) to dorsal and caudal (low) gradient of expression in thalamus.

Structure	Level	Pattern	Image	Note
TS24: hippocampus	Moderate		4j
TS24: dorsal thalamus	Strong		4j

Structure	Level	Pattern	Image	Note
TS24: 3rd ventricle ependyma	Present		4k	Expression in neuroepithelium of 3rd ventricle.

Structure	Level	Pattern	Image	Note
TS24: preoptic area	Moderate		4l

Structure	Level	Pattern	Image	Note
TS24: midbrain tegmentum	Present		4m	Strong expression in mesencephalic tegmentum.
TS24: midbrain	Present	Regionally restricted	4m	Authors state that ventral mesencephalon was unstained.
TS24: cerebral aqueduct	Present	Regionally restricted	4m	Expression in neuroepithelium of aqueduct.

Structure	Level	Pattern	Image	Note
TS24: superior colliculus	Strong		4n
TS24: cerebral aqueduct	Present	Regionally restricted	4n	Expression in neuroepithelium of aqueduct.

Structure	Level	Pattern	Image	Note
TS24: hypothalamus	Strong		4o

Structure	Level	Pattern	Image	Note
TS24: midbrain-hindbrain junction	Moderate		4p	Moderate expression in isthmus.
TS24: pons	Strong		4p
TS24: 4th ventricle ependyma	Present		4p	Expression in neuroepithelium of 4th ventricle.

Structure	Level	Pattern	Image	Note
TS24: 4th ventricle ependyma	Present		4q	Expression in neuroepithelium of 4th ventricle.

Structure	Level	Pattern	Image	Note
TS24: cerebellum	Strong		4r

Structure	Level	Pattern	Image	Note
TS24: telencephalon septum	Strong		4s

Structure	Level	Pattern	Image	Note
TS24: inferior colliculus	Strong	Graded	4t	Caudal (high) to rostral (low) gradient of expression in ventricular zone of inferior colliculus.

Structure	Level	Pattern	Image	Note
TS24: lateral ganglionic eminence	Strong	Graded	4u	Ventral and rostral (high) to dorsal and caudal (low) gradienct of expression.
TS24: prethalamus	Strong	Graded	4u	Ventral and rostral (high) to dorsal and caudal (low) gradient of expression in thalamus.

Structure	Cell Type	Level	Pattern	Image	Note
TS24: retina		Present	Regionally restricted	5	Expression in nasal part of retina.
TS24: retina layer	retinal ganglion cell	Present		5

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb prospective granule cell layer	Moderate		6a

Structure	Level	Pattern	Image	Note
TS26: olfactory cortex	Moderate	Regionally restricted	6b	Moderate expression in olfactory tubercle.
TS26: piriform cortex	Weak

Structure	Level	Pattern	Image	Note
TS26: anterior medial cortex	Weak	Regionally restricted	6c	Weak expression in cingulate cortex.

Structure	Level	Pattern	Image	Note
TS26: rostral migratory stream	Moderate	Regionally restricted	6d	Moderate expression in lateral migratory stream.
TS26: corpus striatum	Weak		6d
TS26: insular cortex	Weak		6d

Structure	Level	Pattern	Image	Note
TS26: frontal cortex	Weak		6e

Structure	Level	Pattern	Image	Note
TS26: telencephalon septum	Moderate	Graded	6f	Moderate expression in septum. Lateral and ventral (high) to medaial and dorsal (low) gradient of expression in septum.

Structure	Level	Pattern	Image	Note
TS26: parietal cortex	Moderate	Regionally restricted	6g	Moderate expression in primary somatosensory region of parietal cortex.

Structure	Level	Pattern	Image	Note
TS26: corpus striatum	Weak		6h
TS26: insular cortex	Weak		6h

Structure	Level	Pattern	Image	Note
TS26: retrosplenial cortex	Weak		6i

Structure	Level	Pattern	Image	Note
TS26: thalamus	Moderate	Graded	6j	Moderate expression in thalamus. Lateral and dorsal (high) to medial and ventral (low) gradient of expression in thalamus.

Structure	Level	Pattern	Image	Note
TS26: 3rd ventricle ependyma	Strong		6k	Strong expression in neuroepithelium of 3rd ventricle.

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Moderate		6l
TS26: hippocampus CA1	Moderate		6l
TS26: hippocampus CA3	Moderate		6l

Structure	Level	Pattern	Image	Note
TS26: occipital cortex	Moderate	Regionally restricted	6m	Moderate expression in layers I and V in occipital cortex.

Structure	Level	Pattern	Image	Note
TS26: perirhinal cortex	Moderate		6n

Structure	Level	Pattern	Image	Note
TS26: superior colliculus	Present	Regionally restricted	6o	Expression in commissure of superior colliculus.

Structure	Level	Pattern	Image	Note
TS26: hypothalamus	Weak		6p
TS26: 3rd ventricle ependyma	Strong		6p	Strong expression in neuroepithelium of 3rd ventricle.

Structure	Level	Pattern	Image	Note
TS26: midbrain tegmentum	Present		6q	Expression in mesencephalic tegmentum.
TS26: midbrain	Present	Regionally restricted	6q	Authors state that ventral mesencephalon was unstained.

Structure	Level	Pattern	Image	Note
TS26: inferior colliculus	Moderate	Regionally restricted	6r	Moderate expression in inferior colliculus. Lateral (high) to medial (low) gradient of expression in inferior colliculus. Expression stronger than in superior region.
TS26: superior colliculus	Weak		6r
TS26: 3rd ventricle ependyma	Strong		6r	Strong expression in neuroepithelium of 3rd ventricle.

Structure	Level	Pattern	Image	Note
TS26: 3rd ventricle ependyma	Strong		6s	Strong expression in neuroepithelium of 3rd ventricle.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Weak		6t
TS26: pons	Weak		6t

Structure	Level	Pattern	Image	Note
TS26: 4th ventricle ependyma	Strong		6u	Strong expression in neuroepithelium of 4th ventricle.

Structure	Level	Pattern	Image	Note
TS26: corpus striatum	Weak		6v
TS26: accumbens nucleus	Trace		6v	Expression almost non-existent in nucleus accumbens.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb granule cell layer	Weak		7a

Structure	Level	Pattern	Image	Note
TS27: olfactory cortex	Weak	Regionally restricted	7b	Weak expression in olfactory subventricular zone.

Structure	Level	Pattern	Image	Note
TS27: frontal cortex	Weak		7c

Structure	Level	Pattern	Image	Note
TS27: telencephalon subventricular zone	Weak		7d

Structure	Level	Pattern	Image	Note
TS27: corpus striatum	Weak	Graded	7e	Discrete lateral and ventral (high) to medial and dorsal (low) gradient of expression in striatum.
TS27: parietal cortex	Weak	Graded	7e

Structure	Level	Pattern	Image	Note
TS27: olfactory tubercle	Weak		7f
TS27: olfactory tract	Weak	Regionally restricted	7f	Weak expression in lateral olfactory tract.
TS27: accumbens nucleus	Weak		7f

Structure	Level	Pattern	Image	Note
TS27: retrosplenial cortex	Weak		7g

Structure	Level	Pattern	Image	Note
TS27: parietal cortex	Weak		7h
TS27: hippocampus	Absent		7h

Structure	Level	Pattern	Image	Note
TS27: corpus striatum	Weak		7i
TS27: dorsal thalamus	Trace		7i	Very weak expression in dorsal and ventral parts of thalamus.
TS27: prethalamus	Trace		7i	Very weak expression in dorsal and ventral parts of thalamus.

Structure	Level	Pattern	Image	Note
TS27: superior colliculus	Weak		7j

Structure	Level	Pattern	Image	Note
TS27: superior colliculus	Moderate	Regionally restricted	7k	Moderate expression commissure of superior colliculus.

Structure	Level	Pattern	Image	Note
TS27: corpus striatum	Weak		7l
TS27: prethalamus	Weak		7l	Weak expression in ventral part of thalamus.

Structure	Level	Pattern	Image	Note
TS27: inferior colliculus	Weak		7m	Caudal (high) to dorsal (low) gradient of expression in inferior colliculus.

Structure	Level	Pattern	Image	Note
TS27: cerebellum	Weak		7n

Structure	Level	Pattern	Image	Note
TS27: telencephalon septum	Moderate		7o

Structure	Level	Pattern	Image	Note
TS27: hypothalamus	Moderate		7p

Structure	Level	Pattern	Image	Note
TS27: midbrain tegmentum	Weak		7q	Weak expression in mesencephalic tegmentum.

Structure	Level	Pattern	Image	Note
TS27: occipital cortex	Absent			Piriform, occipital and perirhinal cortices were unstained.
TS27: perirhinal cortex	Absent			Piriform, occipital and perirhinal cortices were unstained.
TS27: piriform cortex	Absent			Piriform, occipital and perirhinal cortices were unstained.
TS27: 3rd ventricle ependyma	Absent			Ventricle and Sylvius aqueduct's neuroepithelia wre unstained.
TS27: 4th ventricle ependyma	Absent			Ventricle and Sylvius aqueduct's neuroepithelia wre unstained.
TS27: cerebral aqueduct	Absent			Ventricle and Sylvius aqueduct's neuroepithelia wre unstained.