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Gene Expression Data
Assay Details
Assay
Reference: J:311215 Fazilaty H, et al., Tracing colonic embryonic transcriptional profiles and their reactivation upon intestinal damage. Cell Rep. 2021 Aug 3;36(5):109484
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7460295
Gene symbol: Lgr5
Gene name: leucine rich repeat containing G protein coupled receptor 5
Reporter: RFP
Antibody: anti-RFP (600-401-379)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2F - E14.5: embryonic day 14.5; Lgr5em1(cre/ERT2)Kba/Lgr5+ (more )
Structure Level Pattern Image Note
TS22: hindgut epithelium Absent 2F Lgr5 E14.5
Specimen 2F - E15.5: embryonic day 15.5; Lgr5em1(cre/ERT2)Kba/Lgr5+ (more )
Structure Level Pattern Image Note
TS23: hindgut epithelium Absent 2F Lgr5 E15.5
Specimen 2F - E18.5: embryonic day 18.5; Lgr5em1(cre/ERT2)Kba/Lgr5+ (more )
Structure Level Pattern Image Note
TS26: hindgut epithelium Present Single cells 2F Lgr5 Inset 1 , 2F Lgr5 E18.5
Specimen 2F - Adult: postnatal adult; Lgr5em1(cre/ERT2)Kba/Lgr5+ (more )
Structure Cell Type Level Pattern Image Note
TS28: large intestine crypt of lieberkuhn intestinal crypt stem cell of colon Present Single cells 2F Lgr5 Adult Expression is seen in stem cells at the bottom of the crypts.
Specimen 2H: embryonic day 15.5; Lgr5em1(cre/ERT2)Kba/Lgr5+ (more )
Structure Level Pattern Image Note
TS23: hindgut epithelium Absent 2H E15.5
Specimen S3E - E16.5: embryonic day 16.5; Lgr5em1(cre/ERT2)Kba/Lgr5+ (more )
Note: Transverse section.
Structure Level Pattern Image Note
TS24: hindgut epithelium Present Single cells S3E E16.5
Specimen S3E - E17.5: embryonic day 17.5; Lgr5em1(cre/ERT2)Kba/Lgr5+ (more )
Note: Transverse section.
Structure Level Pattern Image Note
TS25: hindgut epithelium Present Single cells S3E E17.5
Specimen S3E - P0: postnatal day 0; Lgr5em1(cre/ERT2)Kba/Lgr5+ (more )
Note: Transverse section.
Structure Cell Type Level Pattern Image Note
TS27: large intestine crypt of lieberkuhn intestinal crypt stem cell of colon Present Single cells S3E P0 Expression is seen in stem cells at the bottom of the crypts.
Specimen S3E - P10: postnatal day 10; Lgr5em1(cre/ERT2)Kba/Lgr5+ (more )
Note: Transverse section.
Structure Cell Type Level Pattern Image Note
TS28: large intestine crypt of lieberkuhn intestinal crypt stem cell of colon Present Single cells S3E P10 Expression is seen in stem cells at the bottom of the crypts.
Specimen S3E - P21: postnatal day 21; Lgr5em1(cre/ERT2)Kba/Lgr5+ (more )
Note: Transverse section.
Structure Cell Type Level Pattern Image Note
TS28: large intestine crypt of lieberkuhn intestinal crypt stem cell of colon Present Single cells S3E P21 Expression is seen in stem cells at the bottom of the crypts.
Specimen S3H: embryonic day 14.5; Lgr5em1(cre/ERT2)Kba/Lgr5+ (more )
Note: Transverse section.
Structure Level Pattern Image Note
TS22: hindgut epithelium Absent S3H E14.5
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory