Antibody:

Anti-CD31 (ab28364)

Structure	Level	Pattern	Image	Note
TS22: skin	Present		3d WT

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	3d KO	Expression in small broken circles of cells, suggesting failed vessels.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: dermis	dermis blood vessel endothelial cell	Present	Regionally restricted	3e WT	Expression in capillaries in skin.

Structure	Level	Pattern	Image	Note
TS22: dermis	Present	Regionally restricted	3e KO	Expression in misshapen subcutaneous structures and broken lines suggesting capillaries or larger vessels had degraded.

Structure	Level	Pattern	Image	Note
TS22: hand	Trace		S3_CD31	Authors state forepaws develop normally except for being smaller, pale and lacking intact blood vessels.

Structure	Level	Pattern	Image	Note
TS22: hand	Trace	Regionally restricted	S3_CD31 inset	Expression in a few nuclei partially surrounded by stained endothelial cell material.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: lung	lung endothelial cell	Present		4a CD31 WT

Structure	Level	Pattern	Image	Note
TS22: lung	Trace	Regionally restricted	4a CD31 KO	Authors report expression almost absent.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: lung vascular element	lung microvascular endothelial cell	Strong	Regionally restricted	4b CD31 WT	Strong expression in pulmonary vascular endothelial cells lining surfaces of lung vasculature.

Structure	Level	Pattern	Image	Note
TS22: lung vascular element	Trace	Regionally restricted	4b CD31 KO	Limited staining appear to be cell fragments of necrotic vessel wall endothelial cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: bronchiole epithelium	epithelial cell of tracheobronchial tree	Present		4c CD31 WT

Structure	Level	Pattern	Image	Note
TS22: bronchiole	Present	Regionally restricted	4c CD31 KO	Authors report bronchioles are smaller and/or degraded. Lung appears to be fragmenting along the paths of failed vessels.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: liver vascular element	endothelial cell of hepatic sinusoid	Present		4d CD31 WT

Structure	Level	Pattern	Image	Note
TS22: liver	Trace	Regionally restricted	4d CD31 KO	Authors report expression almost absent. Liminted staining appear to be cell fragments of necrotic vessel wall endothelial cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: liver vascular element	endothelial cell of hepatic sinusoid	Present		4e CD31 WT

Structure	Level	Pattern	Image	Note
TS22: liver	Trace	Regionally restricted	4e CD31 KO	Authors report possible fragments of phagocytized cells.

Structure	Level	Pattern	Image	Note
TS22: liver	Trace	Regionally restricted	S4a	Authors report cell fragments and phagocytosis of endothelial cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: heart blood vessel	cardiac blood vessel endothelial cell	Present	Regionally restricted	5a CD31 WT	Expression in endothelial cells of capillaries and larger vessels.

Structure	Level	Pattern	Image	Note
TS22: heart	Trace	Regionally restricted	5a CD31 KO

Structure	Cell Type	Level	Pattern	Image	Note
TS22: heart blood vessel	capillary endothelial cell	Present		5b CD31 WT	Expression in myocardium capillaries.

Structure	Level	Pattern	Image	Note
TS22: heart	Trace	Regionally restricted	5b CD31 KO	Authors report missing myocardium capillaries. Staining in broken circles and lines of misshapen endothelial cells and endothelial cell fragments.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: heart blood vessel	endothelial cell of coronary artery	Present		5c CD31 WT	Expression in intact coronary vessel.

Structure	Level	Pattern	Image	Note
TS22: heart	Trace	Regionally restricted	5c CD31 KO	Authors report degrading coronary vessel beneath epicardium.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: endocardial lining	endocardial cell	Present	Regionally restricted	5e CD31 WT	Expression in intact epithelial cells in endocardium.

Structure	Level	Pattern	Image	Note
TS22: endocardial lining	Trace	Regionally restricted	5e CD31 KO	Authors report degraded epithelial cells.

Structure	Level	Pattern	Image	Note
TS22: heart	Trace		S4b CD31

Structure	Level	Pattern	Image	Note
TS22: heart	Present	Regionally restricted	S4b CD31 inset (left)	Authors report cell fragments and phagocytosis of endothelial cells.

Structure	Level	Pattern	Image	Note
TS22: heart	Present	Regionally restricted	S4b CD31 inset (right)	Authors report cell fragments and phagocytosis of endothelial cells.