Lgr5 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:277739 Chaves-Perez A, et al., URI is required to maintain intestinal architecture during ionizing radiation. Science. 2019 May 31;364(6443)
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7450887
Gene symbol:	Lgr5
Gene name:	leucine rich repeat containing G protein coupled receptor 5

Reporter:	GFP
Antibody:	anti-GFP (Aves)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

Specimen S16A: postnatal week 8; Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺ (more

)
Note: Double labeled: green - Lgr5; red - Uri1 (assay MGI:7450884).

Structure	Level	Pattern	Image	Note
TS28: crypt of lieberkuhn	Present	Regionally restricted	S16A Merge , S16A Lgr5-EGFP	Expression is seen at the base of the crypts.

Specimen S15E: postnatal week 8; Lgr5^{tm1(cre/ERT2)Cle}/Lgr5⁺ (more

)
Note: Double labeled: green - Lgr5; red - Uri1 (assay MGI:7450884).

Structure	Level	Pattern	Image	Note
TS28: crypt of lieberkuhn	Present	Regionally restricted	S16E Merge , S16E Lgr5-EGFP	Expression is seen at the base of the crypts.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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