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Gene Expression Data
Assay Details
Assay
Reference: J:276168 Bhattacharyya S, et al., Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition. Sci Rep. 2019 Feb 14;9(1):2106
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7449365
Gene symbol: Hcn4
Gene name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Antibody: anti-HCN4 (APC-052)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3a: postnatal day 0; Gjd3tm1.1(cre/EGFP)Nmun/Gjd3+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Double labeled: green - Hcn4; red - Cre reporter.
Structure Level Pattern Image Note
TS27: atrioventricular node Present 3a (iii) , 3a (i)
Specimen 3c - i: postnatal day 28; Gjd3tm1.1(cre/EGFP)Nmun/Gjd3+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: magenta - Hcn4; green - Gjd3 (assay MGI:7449374); red - Cre reporter.
Structure Level Pattern Image Note
TS28: atrioventricular node Present 3c (i) HCN4 , 3c (i) Merged Expression is seen in the distal node.
Specimen 3c - ii: postnatal day 28; Gjd3tm1.1(cre/EGFP)Nmun/Gjd3+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: magenta - Hcn4; green - Gjd3 (assay MGI:7449374); red - Cre reporter.
Structure Level Pattern Image Note
TS28: atrioventricular node Present 3c (ii) Merged , 3c (ii) HCN4 Expression in seen in the nodal extension.
Specimen S8c - Wild-type: postnatal day 42 (more )
Note: Triple labeled: magenta - Hcn4; green - Gjd3 (assay MGI:7449374); red - Cre reporter.
Structure Level Pattern Image Note
TS28: atrioventricular node Present S8c upper HCN4 , S8c upper Merged
Specimen S8c - Het: postnatal day 42; Gjd3tm1.1(cre/EGFP)Nmun/Gjd3+, Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/Gt(ROSA)26Sor+ (more )
Note: Conditional mutant. Triple labeled: magenta - Hcn4; green - Gjd3 (assay MGI:7449374); red - Cre reporter.
Structure Level Pattern Image Note
TS28: atrioventricular node Present S8c lower Merged , S8c lower HCN4 Expression in the het is indistinguishable from that seen in wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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