Gene Expression Data
Assay Details
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Assay
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Reference:
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J:333334
Mayo F, et al., Aquaporin-4 Expression Switches from White to Gray Matter Regions during Postnatal Development of the Central Nervous System. Int J Mol Sci. 2023 Feb 3;24(3)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7442956
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Gene symbol:
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Aqp4
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Gene name:
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aquaporin 4
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Probe:
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Mm-Aqp4 (417161)
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Probe preparation:
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Antisense labelled with other - see notes
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Visualized with:
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Chromogenic
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Assay notes:
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Probe detected with Brown RNAscope 2.5 HD detection kit (ACD #322310),
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Results
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Specimen
S1:
embryonic day 16.0
(more  )
Specimen
S1:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: brainstem |
Present |
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S1
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Authors report incipient expression in brainstem.
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Specimen
1A:
postnatal day 1
(more )
Specimen
1A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: brain |
Weak |
Regionally restricted |
1A
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Expression is scarce, except for brainstem.
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| TS27: brainstem |
Present |
Regionally restricted |
1A
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Expression mainly in area closest to edges.
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Specimen
1B:
postnatal day 3
(more )
Specimen
1B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: diencephalon |
Present |
Regionally restricted |
1B
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Expression in diencephalon more intense than at P1, with increased expression towards pial borders.
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Specimen
1C:
postnatal day 7
(more )
Specimen
1C:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain |
Present |
Regionally restricted |
1C
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Increased abundance and distribution of expression, mainly in glial limitans zones.
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| TS28: cerebral cortex |
Present |
Regionally restricted |
1C
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Expression around entire cortex, and much more extended in dorsal and rostral cortical area.
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| TS28: cerebellum |
Strong |
Regionally restricted |
1C
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Intense expression in cerebellum, concentrating on external border or crest, with especially intense expression in deep layers of crest.
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Specimen
1C':
postnatal day 7
(more )
Specimen
1C':
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1D:
postnatal day 56
(more )
Specimen
1D:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 56
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brain |
Present |
Regionally restricted |
1D
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Expression glia limitans.
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| TS28: lateral ventricle ependyma |
Strong |
Regionally restricted |
1D
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Intense expression in ependymal cells in ventricle.
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| TS28: cerebral cortex |
Present |
Regionally restricted |
1D
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Expression in external layers of cortex.
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| TS28: cerebellum |
Present |
Regionally restricted |
1D
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Expression in outer layers of crest. No expression in most internal white matter zone of crest.
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Specimen
1D':
postnatal day 56
(more )
Specimen
1D':
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 56
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: corpus callosum |
Weak |
Regionally restricted |
1D'
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Expression disappears almost completely from corpus callosum.
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Analysis Tools