Gene Expression Data
Assay Details
Assay
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Reference:
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J:177404
Schneider M, et al., Cell-specific detection of microRNA expression during cardiomyogenesis by combined in situ hybridization and immunohistochemistry. J Mol Histol. 2011 Aug;42(4):289-99
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7442492
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Gene symbol:
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Mir125b-2
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Gene name:
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microRNA 125b-2
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Probe:
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miR-125b probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Rhodamine
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Assay notes:
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The mature microRNA is encoded at multiple sites in the genome.
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Results
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Specimen
3 miR-125b:
embryonic day 14.5
(more )
Note: Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
Specimen
3 miR-125b:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: heart |
Present |
Regionally restricted |
3_miR-125b
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Expression was widely distributed within connective tissues of the heart.
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TS22: dorsal aorta |
Present |
Regionally restricted |
3_miR-125b
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Expression was localized to the aortic wall.
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TS22: atrioventricular septal complex |
Present |
Regionally restricted |
3_miR-125b
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Expression was detected in streaks of connective tissue in the atrioventricular junction.
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Specimen
4b:
postnatal day 98
(more )
Note: Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
Specimen
4b:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 98
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
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Specimen
4e:
postnatal day 98
(more )
Note: Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
Specimen
4e:
(close )
Genetic Background:
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C57BL/6
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Age:
|
postnatal day 98
|
Sex:
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Not Specified
|
Type:
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section
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Fixation:
|
Formalin
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
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Specimen
4h:
postnatal day 98
(more )
Note: Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
Specimen
4h:
(close )
Genetic Background:
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C57BL/6
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Age:
|
postnatal day 98
|
Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
Formalin
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Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
|
Specimen
4k:
postnatal day 98
(more )
Note: Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
Specimen
4k:
(close )
Genetic Background:
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C57BL/6
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Age:
|
postnatal day 98
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
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Structure |
Level |
Pattern |
Image |
Note |
TS28: visceral pericardium |
Present |
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4kR+B+G
,
4kR+B
,
4kR
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Expression was detected in epicardial cells.
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Specimen
4n:
postnatal day 98
(more )
Note: Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
Specimen
4n:
(close )
Genetic Background:
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C57BL/6
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Age:
|
postnatal day 98
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Mir125b-1 and Mir125b-2; green - Myosin.
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Specimen
5 center:
postnatal day 98
(more )
Note: Triple labeled: red - Mir125b-1 and Mir125b-2; green - Acta2 (assay MGI:7442567); magenta - Col1a1 (assay MGI:7442568) and Col1a2 (assay MGI:7442583).
Specimen
5 center:
(close )
Genetic Background:
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C57BL/6
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Age:
|
postnatal day 98
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
Formalin
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Mir125b-1 and Mir125b-2; green - Acta2 (assay MGI:7442567); magenta - Col1a1 (assay MGI:7442568) and Col1a2 (assay MGI:7442583).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: extracellular matrix |
|
Present |
Regionally restricted |
5_miR-125b
,
5_merge center
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In the heart ventricle, expression localized in Acta2-negative perivascular cells embedded in the Collagen-I-positive extracellular matrix .
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TS28: heart ventricle |
smooth muscle cell |
Absent |
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5_miR-125b
,
5_merge center
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Acta2-positive smooth muscle cells showed no expression.
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Specimen
Table 1 E14.5:
embryonic day 14.5
(more )
Specimen
Table 1 E14.5:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS22: cardiac muscle tissue |
cardiac muscle cell |
Weak |
|
|
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TS22: heart |
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Strong |
Regionally restricted |
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Strong expression was detected in connective tissue.
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TS22: connective tissue |
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Strong |
Regionally restricted |
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Strong expression was detected in connective tissue of several organs.
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Specimen
Table 1 P98:
postnatal day 98
(more )
Specimen
Table 1 P98:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 98
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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