Antibody:	Anti-GRP-78
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: Meckel's cartilage	Present		1A

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Present		1B

Structure	Level	Pattern	Image	Note
TS24: jaw bone	Present	Regionally restricted	1C	Expression was detected in alveolar bone.
TS24: Meckel's cartilage	Weak		1C

Structure	Cell Type	Level	Pattern	Image	Note
TS24: incisor	preodontoblast	Present		1D
TS24: tooth basement membrane		Present		1D
TS24: cervical loop		Strong	Regionally restricted	1D	Strong expression was seen in the undifferentiated cells of the cervical loop.
TS24: jaw bone		Strong	Regionally restricted	1D	Expression was detected in the alveolar bone.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: incisor	ameloblast	Present		2A	Localization was predominant in the cytoplasm.
TS27: incisor	odontoblast	Present		2A	Localization was predominant in the cytoplasm.
TS27: incisor	osteoblast	Present		2A	Localization was predominant in the cytoplasm.
TS27: alveolar process		Present		2A
TS27: predentin		Present		2A
TS27: Meckel's cartilage	chondrocyte	Present		2A

Structure	Cell Type	Level	Pattern	Image	Note
TS27: molar	preameloblast	Present		2B
TS27: molar	preodontoblast	Present		2B

Structure	Cell Type	Level	Pattern	Image	Note
TS27: incisor	ameloblast	Present		2C	Localization was predominant in the cytoplasm.
TS27: incisor	odontoblast	Present		2C	Localization was predominant in the cytoplasm.
TS27: incisor	osteoblast	Present		2C	Localization was predominant in the cytoplasm.
TS27: alveolar process		Present		2C
TS27: predentin		Present		2C
TS27: Meckel's cartilage	hypertrophic chondrocyte	Present		2C

Structure	Cell Type	Level	Pattern	Image	Note
TS27: molar	preameloblast	Present		2D
TS27: molar	preodontoblast	Present		2D

Structure	Level	Pattern	Image	Note
TS28: dentin	Present		3A	Expression in the dentin matrix of the incisor.
TS28: alveolar process	Present		3A

Structure	Level	Pattern	Image	Note
TS28: dental pulp	Present	Regionally restricted	3B	A defined population of dental pulp cells showed expression.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dental pulp		Present	Uniform	3C
TS28: dentin		Present	Uniform	3C
TS28: jaw bone		Present	Uniform	3C
TS28: molar	ameloblast	Present		3C

Structure	Cell Type	Level	Pattern	Image	Note
TS28: dental pulp		Present	Uniform	3D
TS28: dentin		Present	Uniform	3D
TS28: jaw bone		Present	Uniform	3D
TS28: molar	ameloblast	Present		3D

Structure	Cell Type	Level	Pattern	Image	Note
TS28: periodontal ligament		Present		4A
TS28: dentin		Present		4A , 4B
TS28: dental pulp	odontoblast	Absent		4B , 4A

Structure	Level	Pattern	Image	Note
TS28: alveolar process	Present		4C
TS28: periodontal ligament	Present		4C

Structure	Level	Pattern	Image	Note
TS27: long bone	Present		5A
TS27: extracellular matrix	Present	Regionally restricted	5A	Expression was detected in the extracellular matrix between the chondrocytes.

Structure	Level	Pattern	Image	Note
TS27: long bone	Present		5B
TS27: extracellular matrix	Present	Regionally restricted	5B	Expression was detected in the extracellular matrix between the chondrocytes.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone		Present	Regionally restricted	5C	Expression was detected in the bone matrix.
TS28: long bone	osteoblast	Present		5C
TS28: extracellular matrix		Present	Regionally restricted	5C	Expression was detected in the cartilage extracellular matrix.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone		Present	Regionally restricted	5D	Expression was detected in the bone matrix.
TS28: long bone	osteoblast	Present		5D
TS28: long bone	osteocyte	Present	Regionally restricted	5D	Expression was seen in the osteocytes embedded within the cortical bone.
TS28: extracellular matrix		Present	Regionally restricted	5D	Expression was detected in the cartilage extracellular matrix.

Structure	Level	Pattern	Image	Note
TS28: long bone	Present	Regionally restricted	5E	Expression was detected in the bony spicules of the trabeculae and in the cortical bone matrix. No staining was observed in the cartilage matrix.
TS28: periosteum	Present		5E

Structure	Level	Pattern	Image	Note
TS28: long bone	Present	Regionally restricted	5F	Expression was detected in the bony spicules of the trabeculae and in the cortical bone matrix. No staining was observed in the cartilage matrix.
TS28: periosteum	Present		5F

Structure	Cell Type	Level	Pattern	Image	Note
TS28: mandible	odontoblast	Present		9E

Structure	Cell Type	Level	Pattern	Image	Note
TS27: alveolar process		Present		S1A
TS27: predentin		Present		S1A
TS27: incisor	odontoblast	Present		S1A

Structure	Level	Pattern	Image	Note
TS28: dentin	Present		S1B

Structure	Cell Type	Level	Pattern	Image	Note
TS27: alveolar process	hypertrophic chondrocyte	Present		S1C

Structure	Cell Type	Level	Pattern	Image	Note
TS28: alveolar process	hypertrophic chondrocyte	Present		S1D

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone		Present	Regionally restricted	S2A	Expression was detected in the bone matrix.
TS28: long bone	osteoblast	Present		S2A
TS28: extracellular matrix		Present		S2A

Structure	Cell Type	Level	Pattern	Image	Note
TS28: long bone		Present	Regionally restricted	S2B	Expression was detected in the bone matrix.
TS28: long bone	osteoblast	Present		S2B
TS28: extracellular matrix		Present		S2B