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Gene Expression Data
Assay Details
Assay
Reference: J:263013 Li Y, et al., Genetic targeting of Purkinje fibres by Sema3a-CreERT2. Sci Rep. 2018 Feb 5;8(1):2382
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7439847
Gene symbol: Sema3a
Gene name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
Reporter: ESR
Antibody: anti-ESR (ab27595)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1C - E12.5: embryonic day 12.5; Sema3atm1.1(cre/ERT2)Bzsh/Sema3a+ (more )
Note: Double labeled: red - Sema3a reporter; green - Tnni3 (assay MGI:7441941).
Structure Cell Type Level Pattern Image Note
TS20: trabecular myocardium cardiac muscle cell Present 1c ESR E12.5 , 1c Merge E12.5
TS20: compact myocardium Trace 1c ESR E12.5 , 1c Merge E12.5
Specimen 1C - E14.5: embryonic day 14.5; Sema3atm1.1(cre/ERT2)Bzsh/Sema3a+ (more )
Note: Double labeled: red - Sema3a reporter; green - Tnni3 (assay MGI:7441941).
Structure Cell Type Level Pattern Image Note
TS22: trabecular myocardium cardiac muscle cell Present 1c ESR E14.5 , 1c Merge E14.5
TS22: compact myocardium Trace 1c Merge E14.5 , 1c ESR E14.5
Specimen 1C - E19.5: embryonic day 19.5; Sema3atm1.1(cre/ERT2)Bzsh/Sema3a+ (more )
Note: Double labeled: red - Sema3a reporter; green - Tnni3 (assay MGI:7441941).
Structure Cell Type Level Pattern Image Note
TS26: trabecular myocardium cardiac muscle cell Present Regionally restricted 1c ESR E19.5 , 1c Merge E19.5 Expression is seen in a subset of subendocardial trabecular cardiomyocytes that are located along the side of the ventricular chamber.
TS26: compact myocardium cardiac muscle cell Absent 1c Merge E19.5 , 1c ESR E19.5 There is no expression in the cardiomyocytes or the coronary arteries of the compact myocardium.
TS26: heart blood vessel Absent 1c ESR E19.5 , 1c Merge E19.5 There is no expression in the cardiomyocytes or the coronary arteries of the compact myocardium.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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