Yap1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:237224 Kim JY, et al., Yap is essential for retinal progenitor cell cycle progression and RPE cell fate acquisition in the developing mouse eye. Dev Biol. 2016 Nov 15;419(2):336-347
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7437580
Gene symbol:	Yap1
Gene name:	yes-associated protein 1

Antibody:	Anti-Phosph-Yap (4911)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

1H ©

1H1 ©

Specimen 1H/H1: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present		1H , 1H1	Expressed at the apical and basal surfaces, in the cytoplasm of the epithelial cells and in the processes.

2A ©

2C ©

Specimen 2A/C: embryonic day 15.5 (more

)
Note: Double labeled: green - Yap1; red - Ctnna1 (assay MGI:7437585).

Structure	Cell Type	Level	Pattern	Image	Note
TS23: pigmented retinal epithelium	retinal pigment epithelial cell	Present		2C , 2A	Partially apical colocalization with Ctnna1 was seen.

5G ©

5G1 ©

5G2 ©

Specimen 5G: embryonic day 16.5; Tg(rx3-icre)1Mjam/0, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant. Double labeled: green - Yap1; red - Prkci (assay MGI:7437616).

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	5G1 , 5G , 5G2	Partial disruption of Prkci expression in most, but not all, retinal areas without Yap1 expression at the apical surface.

5H ©

5H1 ©

5H2 ©

Specimen 5H: embryonic day 15.5; Tg(rx3-icre)1Mjam/0, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant. Double labeled: green - Yap1; red - Ctnna1 (assay MGI:7437585).

Structure	Level	Pattern	Image	Note
TS23: neural retina	Present	Regionally restricted	5H2 , 5H , 5H1	Yap1 devoid retinal patches were seen.
TS23: pigmented retinal epithelium	Present	Regionally restricted	5H2 , 5H , 5H1	Yap1 devoid retinal patches were seen.

S3C ©

Specimen S3C: embryonic day 12.5; Tg(rx3-icre)1Mjam/0, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: neural retina	Present	Regionally restricted	S3C	Yap1 devoid retinal patches were more frequent in central than peripheral retina.

S3G ©

Specimen S3G: postnatal day 0; Tg(rx3-icre)1Mjam/0, Yap1^tm1.1Eno/Yap1^tm1.1Eno (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present	Regionally restricted	S3G	Yap1 devoid retinal patches were more frequent in central than peripheral retina.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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