Probe:	Ptch1 probe
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Graded	1B	Expression was in a graded pattern, more strongly on the ventral side and becoming gradually weaker toward the dorsal side.
TS15: otocyst	Present	Graded	1B	Expression was in a graded pattern, more strongly on the ventral side and becoming gradually weaker toward the dorsal side.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Graded	1G	Expression was in a graded pattern, more strongly on the ventral side and becoming gradually weaker toward the dorsal side.
TS17: otocyst	Present	Graded	1G	Expression was in a graded pattern, more strongly on the ventral side and becoming gradually weaker toward the dorsal side.

Structure	Level	Pattern	Image	Note
TS20: inner ear	Present	Graded	2C	Expression was in graded pattern, more weakly in the dorsal vestibular organs and becoming gradually stronger toward the ventral cochlea.
TS20: cochlea	Present	Graded	2C
TS20: inner ear vestibular component	Present	Graded	2C

Structure	Level	Pattern	Image	Note
TS20: inner ear	Present	Graded	2I	Expression was in graded pattern, more weakly in the dorsal vestibular organs and becoming gradually stronger toward the ventral cochlea.
TS20: cochlea	Present	Graded	2I
TS20: inner ear vestibular component	Present	Graded	2I

Structure	Level	Pattern	Image	Note
TS20: inner ear	Present	Graded	2O	Expression was in graded pattern, more weakly in the dorsal vestibular organs and becoming gradually stronger toward the ventral cochlea.
TS20: cochlea	Present	Graded	2O
TS20: inner ear vestibular component	Present	Graded	2O

Structure	Level	Pattern	Image	Note
TS20: inner ear	Present	Graded	2U	Expression was in graded pattern, more weakly in the dorsal vestibular organs and becoming gradually stronger toward the ventral cochlea.
TS20: cochlea	Present	Graded	2U
TS20: inner ear vestibular component	Present	Graded	2U
TS20: vestibulo-cochlear ganglion	Present		2U

Structure	Level	Pattern	Image	Note
TS20: vestibulo-cochlear ganglion	Present	Regionally restricted	3D	Expression was upregulated only at the cochlear level of the cochleovestibular ganglion.

Structure	Level	Pattern	Image	Note
TS20: vestibulo-cochlear ganglion	Present	Regionally restricted	3I	Expression was upregulated only at the cochlear level of the cochleovestibular ganglion.

Structure	Level	Pattern	Image	Note
TS23: inner ear vestibular component	Absent		4C

Structure	Cell Type	Level	Pattern	Image	Note
TS23: cochlea epithelium		Present	Graded	4D' , 4D	Expression was stronger in the apex and weaker toward the base.
TS23: inner ear mesenchyme		Present	Graded	4D' , 4D	Expression was stronger in the apex and weaker toward the base.
TS23: cochlear ganglion	spiral ganglion neuron	Present		4D , 4D'

Structure	Level	Pattern	Image	Note
TS27: inner ear	Absent		5C' , 5C'' , 5C

Structure	Level	Pattern	Image	Note
TS27: inner ear	Absent		5D' , 5D'' , 5D

Structure	Level	Pattern	Image	Note
TS23: middle ear	Present	Regionally restricted	6C	Expression was detected in middle ear joints and annular ligaments.
TS23: stapes	Present	Regionally restricted	6C	Expression was detected in the stapedial footplate.

Structure	Level	Pattern	Image	Note
TS23: middle ear	Present	Regionally restricted	6I	Expression was detected in middle ear joints.