Slc12a2 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:282974 Lee M, et al., Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. J Cell Biol. 2020 Feb 3;219(2)
Assay type:	Western blot
MGI Accession ID:	MGI:7435289
Gene symbol:	Slc12a2
Gene name:	solute carrier family 12, member 2

Antibody:	anti-NKCC1 (N-16) (sc-21545)
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. Anti-NKCC1 (T4) MGI:7435240 may have been used in this assay.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Genetic Background	Mutant Allele(s)	Sex
E16 +/+	E16.0 (a)	TS24: cerebral cortex	Present (b)	involves: C57BL/6		Not Specified
P1 +/+	P1	TS27: cerebral cortex	Present (b)	involves: C57BL/6		Not Specified
P4 +/+	P4	TS28: cerebral cortex	Weak (c)	involves: C57BL/6		Not Specified
P7 +/+	P4	TS28: cerebral cortex	Weak (c)	involves: C57BL/6		Not Specified
E16. -/-	E16.0 (a)	TS24: cerebral cortex	Strong (d)	C57BL/6-Ecpas^em1Plch	Ecpas^em1Plch/Ecpas^em1Plch	Not Specified
P1 -/-	P1	TS27: cerebral cortex	Strong (d)	C57BL/6-Ecpas^em1Plch	Ecpas^em1Plch/Ecpas^em1Plch	Not Specified
P4 -/-	P4	TS28: cerebral cortex	Present (e)	C57BL/6-Ecpas^em1Plch	Ecpas^em1Plch/Ecpas^em1Plch	Not Specified
P7 -/-	P4	TS28: cerebral cortex	Present (e)	C57BL/6-Ecpas^em1Plch	Ecpas^em1Plch/Ecpas^em1Plch	Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression at higher levels at embryonic and postnatal days before P4.
(c) Expression gradually decreased from P4.
(d) Signficantly higher expression compared with wildtype.
(e) Slower rate of decline compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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