Calb1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:332658 Keeley PW, et al., Neurog2 regulates Isl1 to modulate horizontal cell number. Development. 2023 Jan 1;150(1):dev201315
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7431226
Gene symbol:	Calb1
Gene name:	calbindin 1

Antibody:	anti-Calbindin D-28K (Ab-1) (PC253L)
Detection system:	Secondary antibody
Assay notes:	Control mice were littermates lacking either floxed or Chx10-cre alleles.

Results

3B CTRL ©

Specimen 3B CTRL: postnatal week 6-8 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present		3B CTRL

3B CKO ©

Specimen 3B CKO: postnatal week 6-8; Neurog2^{tm5(Neurog2)Fgu}/Neurog2^{tm5(Neurog2)Fgu}, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present		3B CKO	Significantly fewer horizontal cells compared to control.

3E CTRL ©

Specimen 3E CTRL: postnatal week 6-8 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present		3E CTRL

3E CKO ©

Specimen 3E CKO: postnatal week 6-8; Neurog2^{tm5(Neurog2)Fgu}/Neurog2^{tm5(Neurog2)Fgu}, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present	Regionally restricted	3E CKO	Characteristic expression in stratified horizontal cell somata and processes.

5B CTRL ©

Specimen 5B CTRL: postnatal day 5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present		5B CTRL

5B DCKO ©

Specimen 5B DCKO: postnatal day 5; Isl1^tm2Sev/Isl1^tm2Sev, Neurog2^{tm5(Neurog2)Fgu}/Neurog2^{tm5(Neurog2)Fgu}, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present		5B DCKO	Significant increase in horizontal cell number.

5E CTRL ©

Specimen 5E CTRL: postnatal day 5 (more

)

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present		5E CTRL

5E DCKO ©

Specimen 5E DCKO: postnatal day 5; Isl1^tm2Sev/Isl1^tm2Sev, Neurog2^{tm5(Neurog2)Fgu}/Neurog2^{tm5(Neurog2)Fgu}, Tg(Chx10-EGFP/cre,-ALPP)2Clc/0 (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: retina	retina horizontal cell	Present	Regionally restricted	5E DCKO	Stratification and differentation of horizontal cells appeared unaltered.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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