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Gene Expression Data
Assay Details
Assay
Reference: J:332642 Cohen-Gulkar M, et al., The LHX2-OTX2 transcriptional regulatory module controls retinal pigmented epithelium differentiation and underlies genetic risk for age-related macular degeneration. PLoS Biol. 2023 Jan;21(1):e3001924
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7431194
Gene symbol: Otx2
Gene name: orthodenticle homeobox 2
Antibody: anti-Otx2 (ab21990)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 1G: embryonic day 12.5; Lhx2tm1Monu/Lhx2tm1Monu (more )
Note: Double labeled: red - Otx2; green - Cdh1 (assay MGI:7431195).
Structure Level Pattern Image Note
TS20: pigmented retinal epithelium Present 1G , 1G inset Otx2 , 1G inset merge Normally expressed at this stage.
Specimen 1H: embryonic day 12.5; Lhx2tm1Monu/Lhx2tm1Monu, Tg(Dct-cre)1Apdn/0 (more )
Note: Conditional mutant. Double labeled: red - Otx2; green - Cdh1 (assay MGI:7431195).
Structure Level Pattern Image Note
TS20: pigmented retinal epithelium Weak 1H inset merge , 1H inset Otx2 , 1H Diminished levels of expression.
Specimen 1I: embryonic day 16.5; Lhx2tm1Monu/Lhx2tm1Monu (more )
Note: Double labeled: red - Otx2; green - Cdh1 (assay MGI:7431195).
Structure Level Pattern Image Note
TS24: pigmented retinal epithelium Present 1I inset Otx2 , 1I inset merge , 1I Expression in multilayered epithelium.
Specimen 1J: embryonic day 16.5; Lhx2tm1Monu/Lhx2tm1Monu, Tg(Dct-cre)1Apdn/0 (more )
Note: Conditional mutant. Double labeled: red - Otx2; green - Cdh1 (assay MGI:7431195).
Structure Level Pattern Image Note
TS24: pigmented retinal epithelium Weak 1J , 1J inset Otx2 , 1J inset merge Loss of expression persisted.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory