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Gene Expression Data
Assay Details
Assay
Reference: J:319706 Silachev D, et al., Mouse models characterize GNAO1 encephalopathy as a neurodevelopmental disorder leading to motor anomalies: from a severe G203R to a milder C215Y mutation. Acta Neuropathol Commun. 2022 Jan 28;10(1):9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7425415
Gene symbol: Eomes
Gene name: eomesodermin
Antibody: anti-Eomes/Tbr2 (ab23345)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 4A: embryonic day 18.5 (more )
Note: Double labeled: red - Eomes; green - Nes (assay MGI:7425406).
Structure Level Pattern Image Note
TS26: cerebral cortex subventricular zone Present Regionally restricted 4A Tbr2 , 4A Merged Expression was detected in intermediate progenitors.
TS26: cerebral cortex ventricular zone Present Regionally restricted 4A Tbr2 , 4A Merged Expression was detected in intermediate progenitors.
Specimen 4B: embryonic day 18.5; Gnao1em2Katv/Gnao1+ (more )
Note: Double labeled: red - Eomes; green - Nes (assay MGI:7425406).
Structure Level Pattern Image Note
TS26: cerebral cortex subventricular zone Present 4B Merged , 4B Tbr2 The numbers of immunopositive cells was nearly halved compared to wild type littermates.
TS26: cerebral cortex ventricular zone Present 4B Merged , 4B Tbr2 The numbers of immunopositive cells was nearly halved compared to wild type littermates.
Specimen 4C: embryonic day 18.5; Gnao1em2Katv/Gnao1em2Katv (more )
Note: Double labeled: red - Eomes; green - Nes (assay MGI:7425406).
Structure Level Pattern Image Note
TS26: cerebral cortex subventricular zone Present Regionally restricted 4C Tbr2 , 4C Merged The numbers of immunopositive cells was nearly halved compared to wild type littermates.
TS26: cerebral cortex ventricular zone Present Regionally restricted 4C Tbr2 , 4C Merged The numbers of immunopositive cells was nearly halved compared to wild type littermates.
Specimen S6C +/+: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: frontal cortex Present Regionally restricted S6C +/+ Tbr2 Expression was detected in intermediate neuronal progenitor cells in the motor cortex.
Specimen S6C G203R/+: embryonic day 18.5; Gnao1em1Katv/Gnao1+ (more )
Structure Level Pattern Image Note
TS26: frontal cortex Present Regionally restricted S6C G203R/+ Tbr2 The number of immunopositive intermediate neuronal progenitor cells was decreased compared with wild type.
Specimen S6C G203R/G203R: embryonic day 18.5; Gnao1em1Katv/Gnao1em1Katv (more )
Structure Level Pattern Image Note
TS26: frontal cortex Present Regionally restricted S6C G203R/G203R Tbr2 The number of immunopositive intermediate neuronal progenitor cells was decreased compared with wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory